The Aspergillus nidulans snt genes are required for the regulation of septum formation and cell cycle checkpoints

Peter R. Kraus, Steven D Harris

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In Aspergillus nidulans, germinating conidia undergo multiple rounds of nuclear division before forming a septum. Previous genetic results suggest that the ability to separate nuclear division and septum formation depends upon a threshold level of activity of the cyclin-dependent kinase NIMX,cdk1. Mutations in nimX and nimT, the gene encoding the NIMXcdk1-activating phosphatase, have revealed that Tyr-15 phosphorylation is important for determining the timing of the formation of the first septum. Here, we describe a screen for suppressors of nimT23 (snt), designed to identify additional components of the pathway regulating septum formation. We show that a subset of the snt mutants are defective in the temporal regulation of septum formation and in cell cycle checkpoint responses. Molecular characterization of sntA shows that it is allelic to the previously described ankA gene, which encodes the NIMXcdk1 Tyr-15 kinase. Additional experiments described in this study show that nutritional conditions modulate the timing of septum formation and alter the phenotypes displayed by the snt mutants. A model that suggests that the timing of septum formation is influenced by DNA damage and glucose availability via the sntA and sntB gene products is proposed.

Original languageEnglish (US)
Pages (from-to)557-569
Number of pages13
JournalGenetics
Volume159
Issue number2
StatePublished - Nov 17 2001

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Suppressor Genes
Aspergillus nidulans
Cell Cycle Checkpoints
Cell Nucleus Division
Genes
Fungal Spores
Cyclin-Dependent Kinases
Phosphoric Monoester Hydrolases
DNA Damage
Phosphotransferases
Phosphorylation
Phenotype
Glucose
Mutation

ASJC Scopus subject areas

  • Genetics

Cite this

The Aspergillus nidulans snt genes are required for the regulation of septum formation and cell cycle checkpoints. / Kraus, Peter R.; Harris, Steven D.

In: Genetics, Vol. 159, No. 2, 17.11.2001, p. 557-569.

Research output: Contribution to journalArticle

Kraus, Peter R. ; Harris, Steven D. / The Aspergillus nidulans snt genes are required for the regulation of septum formation and cell cycle checkpoints. In: Genetics. 2001 ; Vol. 159, No. 2. pp. 557-569.
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