The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity

Steven D. Harris, Lisbeth Hamer, Kathryn E. Sharpless, John E. Hamer

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Cytokinesis (septation) in the fungus Aspergillus nidulans occurs through the formation of a transient actin ring at the incipient division site. Temperature-sensitive mutations in the sepA gene prevent septation and cause defects in the maintenance of cellular polarity, without affecting growth and nuclear division. The sepA gene encodes a member of the growing family of FH1/2 proteins, which appear to have roles in morphogenesis and cytokinesis in organisms such as yeast and Drosophila. Results from temperature shift and immunofluorescence microscopy experiments strongly suggest that sepA function requires a preceding mitosis and that sepA acts prior to actin ring formation. Deletion mutants of sepA exhibit temperature-sensitive growth and severe delays in septation at the permissive temperature, indicating that expression of another gene may compensate for the loss of sepA. Conidiophores formed by sepA mutants exhibit abnormal branching of the stalk and vesicle. These results suggest that sepA interacts with the actin cytoskeleton to promote formation of the actin ring during cytokinesis and that sepA is also required for maintenance of cellular polarity during hyphal growth and asexual morphogenesis.

Original languageEnglish (US)
Pages (from-to)3474-3483
Number of pages10
JournalEMBO Journal
Volume16
Issue number12
DOIs
StatePublished - Jun 16 1997

Fingerprint

Aspergillus nidulans
Cytokinesis
Aspergillus
Actins
Genes
Maintenance
Temperature
Morphogenesis
Proteins
Growth
Cell Nucleus Division
Fungi
Actin Cytoskeleton
Fluorescence Microscopy
Mitosis
Yeast
Drosophila
Microscopic examination
Yeasts
Gene Expression

Keywords

  • 2 Protein
  • Aspergillus
  • Cellular polarity
  • Cytokinesis
  • FH1
  • Septation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity. / Harris, Steven D.; Hamer, Lisbeth; Sharpless, Kathryn E.; Hamer, John E.

In: EMBO Journal, Vol. 16, No. 12, 16.06.1997, p. 3474-3483.

Research output: Contribution to journalArticle

Harris, Steven D. ; Hamer, Lisbeth ; Sharpless, Kathryn E. ; Hamer, John E. / The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity. In: EMBO Journal. 1997 ; Vol. 16, No. 12. pp. 3474-3483.
@article{bb3022da6727492f9088b0b0d6cdc8a5,
title = "The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity",
abstract = "Cytokinesis (septation) in the fungus Aspergillus nidulans occurs through the formation of a transient actin ring at the incipient division site. Temperature-sensitive mutations in the sepA gene prevent septation and cause defects in the maintenance of cellular polarity, without affecting growth and nuclear division. The sepA gene encodes a member of the growing family of FH1/2 proteins, which appear to have roles in morphogenesis and cytokinesis in organisms such as yeast and Drosophila. Results from temperature shift and immunofluorescence microscopy experiments strongly suggest that sepA function requires a preceding mitosis and that sepA acts prior to actin ring formation. Deletion mutants of sepA exhibit temperature-sensitive growth and severe delays in septation at the permissive temperature, indicating that expression of another gene may compensate for the loss of sepA. Conidiophores formed by sepA mutants exhibit abnormal branching of the stalk and vesicle. These results suggest that sepA interacts with the actin cytoskeleton to promote formation of the actin ring during cytokinesis and that sepA is also required for maintenance of cellular polarity during hyphal growth and asexual morphogenesis.",
keywords = "2 Protein, Aspergillus, Cellular polarity, Cytokinesis, FH1, Septation",
author = "Harris, {Steven D.} and Lisbeth Hamer and Sharpless, {Kathryn E.} and Hamer, {John E.}",
year = "1997",
month = "6",
day = "16",
doi = "10.1093/emboj/16.12.3474",
language = "English (US)",
volume = "16",
pages = "3474--3483",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity

AU - Harris, Steven D.

AU - Hamer, Lisbeth

AU - Sharpless, Kathryn E.

AU - Hamer, John E.

PY - 1997/6/16

Y1 - 1997/6/16

N2 - Cytokinesis (septation) in the fungus Aspergillus nidulans occurs through the formation of a transient actin ring at the incipient division site. Temperature-sensitive mutations in the sepA gene prevent septation and cause defects in the maintenance of cellular polarity, without affecting growth and nuclear division. The sepA gene encodes a member of the growing family of FH1/2 proteins, which appear to have roles in morphogenesis and cytokinesis in organisms such as yeast and Drosophila. Results from temperature shift and immunofluorescence microscopy experiments strongly suggest that sepA function requires a preceding mitosis and that sepA acts prior to actin ring formation. Deletion mutants of sepA exhibit temperature-sensitive growth and severe delays in septation at the permissive temperature, indicating that expression of another gene may compensate for the loss of sepA. Conidiophores formed by sepA mutants exhibit abnormal branching of the stalk and vesicle. These results suggest that sepA interacts with the actin cytoskeleton to promote formation of the actin ring during cytokinesis and that sepA is also required for maintenance of cellular polarity during hyphal growth and asexual morphogenesis.

AB - Cytokinesis (septation) in the fungus Aspergillus nidulans occurs through the formation of a transient actin ring at the incipient division site. Temperature-sensitive mutations in the sepA gene prevent septation and cause defects in the maintenance of cellular polarity, without affecting growth and nuclear division. The sepA gene encodes a member of the growing family of FH1/2 proteins, which appear to have roles in morphogenesis and cytokinesis in organisms such as yeast and Drosophila. Results from temperature shift and immunofluorescence microscopy experiments strongly suggest that sepA function requires a preceding mitosis and that sepA acts prior to actin ring formation. Deletion mutants of sepA exhibit temperature-sensitive growth and severe delays in septation at the permissive temperature, indicating that expression of another gene may compensate for the loss of sepA. Conidiophores formed by sepA mutants exhibit abnormal branching of the stalk and vesicle. These results suggest that sepA interacts with the actin cytoskeleton to promote formation of the actin ring during cytokinesis and that sepA is also required for maintenance of cellular polarity during hyphal growth and asexual morphogenesis.

KW - 2 Protein

KW - Aspergillus

KW - Cellular polarity

KW - Cytokinesis

KW - FH1

KW - Septation

UR - http://www.scopus.com/inward/record.url?scp=0030927282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030927282&partnerID=8YFLogxK

U2 - 10.1093/emboj/16.12.3474

DO - 10.1093/emboj/16.12.3474

M3 - Article

C2 - 9218790

AN - SCOPUS:0030927282

VL - 16

SP - 3474

EP - 3483

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 12

ER -