The arabidopsis MOS4-associated complex promotes microRNA biogenesis and precursor messenger RNA splicing

Tianran Jia, Bailong Zhang, Chenjiang You, Yong Zhang, Liping Zeng, Shengjun Li, Kaeli C.M. Johnson, Bin Yu, Xin Li, Xuemei Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In Arabidopsis thaliana, the MOS4-ASSOCIATED COMPLEX (MAC) is required for defense and development. The evolutionarily conserved, putative RNA helicase MAC7 is a component of the Arabidopsis MAC, and the human MAC7 homolog, Aquarius, is implicated in pre-mRNA splicing. Here, we show that mac7-1, a partial loss-of-function mutant in MAC7, and two other MAC subunit mutants, mac3a mac3b and prl1 prl2 (pleiotropic regulatory locus), exhibit reduced microRNA (miRNA) levels, indicating that MAC promotes miRNA biogenesis. The mac7-1 mutant shows reduced primary miRNA (pri-miRNA) levels without affecting miRNA gene (MIR) promoter activity or the half-life of pri-miRNA transcripts. As a nuclear protein, MAC7 is not concentrated in dicing bodies, but it affects the localization of HYPONASTIC LEAVES1 (HYL1), a key protein in pri-miRNA processing, to dicing bodies. Immunoprecipitation of HYL1 retrieved 11 known MAC subunits, including MAC7, indicating association between HYL1 and MAC. We propose that MAC7 links MIR transcription to pri-miRNA processing. RNA-seq analysis showed that downregulated genes in MAC subunit mutants are mostly involved in plant defense and stimulus responses, confirming a role of MAC in biotic and abiotic stress responses. We also discovered global intron retention defects in mutants in three subunits of MAC, thus linking MAC function to splicing in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)2626-2643
Number of pages18
JournalPlant Cell
Volume29
Issue number10
DOIs
StatePublished - Oct 2017

Fingerprint

RNA splicing
RNA Splicing
MicroRNAs
microRNA
Arabidopsis
mutants
RNA helicases
RNA Helicases
Genes
genes
biogenesis
nuclear proteins
RNA Precursors
Nuclear Proteins
biotic stress
Immunoprecipitation
Introns
abiotic stress
half life
Half-Life

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Jia, T., Zhang, B., You, C., Zhang, Y., Zeng, L., Li, S., ... Chen, X. (2017). The arabidopsis MOS4-associated complex promotes microRNA biogenesis and precursor messenger RNA splicing. Plant Cell, 29(10), 2626-2643. https://doi.org/10.1105/tpc.17.00370

The arabidopsis MOS4-associated complex promotes microRNA biogenesis and precursor messenger RNA splicing. / Jia, Tianran; Zhang, Bailong; You, Chenjiang; Zhang, Yong; Zeng, Liping; Li, Shengjun; Johnson, Kaeli C.M.; Yu, Bin; Li, Xin; Chen, Xuemei.

In: Plant Cell, Vol. 29, No. 10, 10.2017, p. 2626-2643.

Research output: Contribution to journalArticle

Jia, T, Zhang, B, You, C, Zhang, Y, Zeng, L, Li, S, Johnson, KCM, Yu, B, Li, X & Chen, X 2017, 'The arabidopsis MOS4-associated complex promotes microRNA biogenesis and precursor messenger RNA splicing', Plant Cell, vol. 29, no. 10, pp. 2626-2643. https://doi.org/10.1105/tpc.17.00370
Jia, Tianran ; Zhang, Bailong ; You, Chenjiang ; Zhang, Yong ; Zeng, Liping ; Li, Shengjun ; Johnson, Kaeli C.M. ; Yu, Bin ; Li, Xin ; Chen, Xuemei. / The arabidopsis MOS4-associated complex promotes microRNA biogenesis and precursor messenger RNA splicing. In: Plant Cell. 2017 ; Vol. 29, No. 10. pp. 2626-2643.
@article{2ca08243cb6548a5b31d08efc3718844,
title = "The arabidopsis MOS4-associated complex promotes microRNA biogenesis and precursor messenger RNA splicing",
abstract = "In Arabidopsis thaliana, the MOS4-ASSOCIATED COMPLEX (MAC) is required for defense and development. The evolutionarily conserved, putative RNA helicase MAC7 is a component of the Arabidopsis MAC, and the human MAC7 homolog, Aquarius, is implicated in pre-mRNA splicing. Here, we show that mac7-1, a partial loss-of-function mutant in MAC7, and two other MAC subunit mutants, mac3a mac3b and prl1 prl2 (pleiotropic regulatory locus), exhibit reduced microRNA (miRNA) levels, indicating that MAC promotes miRNA biogenesis. The mac7-1 mutant shows reduced primary miRNA (pri-miRNA) levels without affecting miRNA gene (MIR) promoter activity or the half-life of pri-miRNA transcripts. As a nuclear protein, MAC7 is not concentrated in dicing bodies, but it affects the localization of HYPONASTIC LEAVES1 (HYL1), a key protein in pri-miRNA processing, to dicing bodies. Immunoprecipitation of HYL1 retrieved 11 known MAC subunits, including MAC7, indicating association between HYL1 and MAC. We propose that MAC7 links MIR transcription to pri-miRNA processing. RNA-seq analysis showed that downregulated genes in MAC subunit mutants are mostly involved in plant defense and stimulus responses, confirming a role of MAC in biotic and abiotic stress responses. We also discovered global intron retention defects in mutants in three subunits of MAC, thus linking MAC function to splicing in Arabidopsis.",
author = "Tianran Jia and Bailong Zhang and Chenjiang You and Yong Zhang and Liping Zeng and Shengjun Li and Johnson, {Kaeli C.M.} and Bin Yu and Xin Li and Xuemei Chen",
year = "2017",
month = "10",
doi = "10.1105/tpc.17.00370",
language = "English (US)",
volume = "29",
pages = "2626--2643",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "10",

}

TY - JOUR

T1 - The arabidopsis MOS4-associated complex promotes microRNA biogenesis and precursor messenger RNA splicing

AU - Jia, Tianran

AU - Zhang, Bailong

AU - You, Chenjiang

AU - Zhang, Yong

AU - Zeng, Liping

AU - Li, Shengjun

AU - Johnson, Kaeli C.M.

AU - Yu, Bin

AU - Li, Xin

AU - Chen, Xuemei

PY - 2017/10

Y1 - 2017/10

N2 - In Arabidopsis thaliana, the MOS4-ASSOCIATED COMPLEX (MAC) is required for defense and development. The evolutionarily conserved, putative RNA helicase MAC7 is a component of the Arabidopsis MAC, and the human MAC7 homolog, Aquarius, is implicated in pre-mRNA splicing. Here, we show that mac7-1, a partial loss-of-function mutant in MAC7, and two other MAC subunit mutants, mac3a mac3b and prl1 prl2 (pleiotropic regulatory locus), exhibit reduced microRNA (miRNA) levels, indicating that MAC promotes miRNA biogenesis. The mac7-1 mutant shows reduced primary miRNA (pri-miRNA) levels without affecting miRNA gene (MIR) promoter activity or the half-life of pri-miRNA transcripts. As a nuclear protein, MAC7 is not concentrated in dicing bodies, but it affects the localization of HYPONASTIC LEAVES1 (HYL1), a key protein in pri-miRNA processing, to dicing bodies. Immunoprecipitation of HYL1 retrieved 11 known MAC subunits, including MAC7, indicating association between HYL1 and MAC. We propose that MAC7 links MIR transcription to pri-miRNA processing. RNA-seq analysis showed that downregulated genes in MAC subunit mutants are mostly involved in plant defense and stimulus responses, confirming a role of MAC in biotic and abiotic stress responses. We also discovered global intron retention defects in mutants in three subunits of MAC, thus linking MAC function to splicing in Arabidopsis.

AB - In Arabidopsis thaliana, the MOS4-ASSOCIATED COMPLEX (MAC) is required for defense and development. The evolutionarily conserved, putative RNA helicase MAC7 is a component of the Arabidopsis MAC, and the human MAC7 homolog, Aquarius, is implicated in pre-mRNA splicing. Here, we show that mac7-1, a partial loss-of-function mutant in MAC7, and two other MAC subunit mutants, mac3a mac3b and prl1 prl2 (pleiotropic regulatory locus), exhibit reduced microRNA (miRNA) levels, indicating that MAC promotes miRNA biogenesis. The mac7-1 mutant shows reduced primary miRNA (pri-miRNA) levels without affecting miRNA gene (MIR) promoter activity or the half-life of pri-miRNA transcripts. As a nuclear protein, MAC7 is not concentrated in dicing bodies, but it affects the localization of HYPONASTIC LEAVES1 (HYL1), a key protein in pri-miRNA processing, to dicing bodies. Immunoprecipitation of HYL1 retrieved 11 known MAC subunits, including MAC7, indicating association between HYL1 and MAC. We propose that MAC7 links MIR transcription to pri-miRNA processing. RNA-seq analysis showed that downregulated genes in MAC subunit mutants are mostly involved in plant defense and stimulus responses, confirming a role of MAC in biotic and abiotic stress responses. We also discovered global intron retention defects in mutants in three subunits of MAC, thus linking MAC function to splicing in Arabidopsis.

UR - http://www.scopus.com/inward/record.url?scp=85033692520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033692520&partnerID=8YFLogxK

U2 - 10.1105/tpc.17.00370

DO - 10.1105/tpc.17.00370

M3 - Article

C2 - 28947490

AN - SCOPUS:85033692520

VL - 29

SP - 2626

EP - 2643

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 10

ER -