The antioxidant mimetic, MnTE-2-PyP, reduces intracellular growth of Mycobacterium abscessus

Rebecca E. Oberley-Deegan, Min Lee Young, G. Eli Morey, Danielle M. Cook, Edward D. Chan, James D. Crapo

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Mycobacterium abscessus is a rapidly growing environmental mycobacterium that can cause severe skin, soft tissue, and lung infections. M. abscessus grows inside macrophages, and these cells release a vast number of proinflammatory cytokines in response to infections. The metalloporphyrin, MnTE-2-PyP, is a broad antioxidant that reduces inflammatory cell signaling. Macrophage-like THP-1 cells were infected with M. abscessus in the presence or absence of MnTE-2-PyP. MnTE-2-PyP significantly decreased, in a dose-dependent manner, the number of M. abscessus organisms recovered from infected THP-1 cells 4 and 8 days after infection. Furthermore, when combined with clarithromycin, MnTE-2-PyP additively reduced the number of cells associated with M. abscessus. A mechanism of bacterial growth inhibition by MnTE-2-PyP was then elucidated. It was found that MnTE-2-PyP promoted the survival of infected THP-1 cells and increased fusion of M. abscessus-containing phagosomes with lysosomes.

Original languageEnglish (US)
Pages (from-to)170-178
Number of pages9
JournalAmerican journal of respiratory cell and molecular biology
Volume41
Issue number2
DOIs
StatePublished - Aug 1 2009

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Keywords

  • Antioxidant mimetic
  • MnTE-2-PyP
  • Mycobacterium abscessus
  • THP-1 cells

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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