The amino- and carboxyl-terminal tails of β-catenin reduce its affinity for desmoglein 2

James K. Wahl, Jill E. Nieset, Paula A. Sacco-Bubulya, Tammy M. Sadler, Keith R. Johnson, Margaret J. Wheelock

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Beta-catenin and plakoglobin are members of the armadillo family of proteins and were first identified as components of intercellular adhering junctions. In the adherens junction β-catenin and plakoglobin serve to link classical cadherins to the actin-based cytoskeleton. In the desmosome plakoglobin links the desmosomal cadherins, the desmogleins and the desmocollins, to the intermediate filament cytoskeleton. β-catenin is not a component of the desmosome. Previously we have shown that the central armadillo repeat region of plakoglobin is the site for desmosomal cadherin binding. We hypothesized that the unique amino- and/or carboxyl-terminal ends of β-catenin may regulate its exclusion from the desmosomal plaque. To test this hypothesis we used chimeras between β-catenin and plakoglobin to identify domain(s) that modulate association with desmoglein 2. Chimeric constructs, each capable of associating with classical cadherins, were assayed for association with the desmosomal cadherin desmoglein 2. Addition of either the N- or C-terminal tail of β-catenin to the armadillo repeats of plakoglobin did not interfere with desmoglein 2 association. However, when both β-catenin amino terminus and carboxyl terminus were added to the plakoglobin armadillo repeats, association with desmoglein 2 was diminished. Removal of the first 26 amino acids from this construct restored association. We show evidence for direct protein-protein interactions between the amino- and carboxyl-terminal tails of β-catenin and propose that a sequence in the first 26 amino acids of β-catenin along with its carboxyl-terminal tail decrease its affinity for desmoglein and prevent its inclusion in the desmosome.

Original languageEnglish (US)
Pages (from-to)1737-1745
Number of pages9
JournalJournal of cell science
Volume113
Issue number10
StatePublished - Jun 17 2000

Fingerprint

Desmoglein 2
gamma Catenin
Catenins
Tail
Desmosomal Cadherins
Armadillos
Desmosomes
Cadherins
Desmogleins
Armadillo Domain Proteins
Desmocollins
Adherens Junctions
Amino Acids
Intercellular Junctions
Intermediate Filaments
beta Catenin
Cytoskeleton
Actin Cytoskeleton
Proteins

Keywords

  • Desmoglein
  • N-cadherin
  • Plakoglobin
  • β-catenin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wahl, J. K., Nieset, J. E., Sacco-Bubulya, P. A., Sadler, T. M., Johnson, K. R., & Wheelock, M. J. (2000). The amino- and carboxyl-terminal tails of β-catenin reduce its affinity for desmoglein 2. Journal of cell science, 113(10), 1737-1745.

The amino- and carboxyl-terminal tails of β-catenin reduce its affinity for desmoglein 2. / Wahl, James K.; Nieset, Jill E.; Sacco-Bubulya, Paula A.; Sadler, Tammy M.; Johnson, Keith R.; Wheelock, Margaret J.

In: Journal of cell science, Vol. 113, No. 10, 17.06.2000, p. 1737-1745.

Research output: Contribution to journalArticle

Wahl, JK, Nieset, JE, Sacco-Bubulya, PA, Sadler, TM, Johnson, KR & Wheelock, MJ 2000, 'The amino- and carboxyl-terminal tails of β-catenin reduce its affinity for desmoglein 2', Journal of cell science, vol. 113, no. 10, pp. 1737-1745.
Wahl JK, Nieset JE, Sacco-Bubulya PA, Sadler TM, Johnson KR, Wheelock MJ. The amino- and carboxyl-terminal tails of β-catenin reduce its affinity for desmoglein 2. Journal of cell science. 2000 Jun 17;113(10):1737-1745.
Wahl, James K. ; Nieset, Jill E. ; Sacco-Bubulya, Paula A. ; Sadler, Tammy M. ; Johnson, Keith R. ; Wheelock, Margaret J. / The amino- and carboxyl-terminal tails of β-catenin reduce its affinity for desmoglein 2. In: Journal of cell science. 2000 ; Vol. 113, No. 10. pp. 1737-1745.
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