The amino acid sequence of the eukaryotic DNA [N6-adenine]methyltransferase M·CviBIII, has regions of similarity with the prokaryotic isoschizomer M · TaqI and other DNA [N6-adenine] methyltransferases

Kenneth E. Narva, James L. Van Etten, Barton E. Slatko, Jack S. Benner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The sequences of the genes coding for M · CviBIII (from virus NC-1A which infects a eukaryotic alga) [Narva et al., Nucleic Acids Res. 15 (1987) 9807-9823] and M·TaqI (from the bacterium Thermus aquaticus) [Slatko et al., Nucleic Acids Res. 15 (1987) 9781-9796] have been determined recently. Both enzymes methylate adenine in the sequence TCGA. We have compared the predicted amino acid sequences of these two methyltransferases (MTases), with each other and with ten other N6A-MTases and find regions of similarity. M · CviBIII and M · TaqI were most closely related followed by M · PaeR7, whose recognition sequence (CTCGAG) contains the M . TaqI/M · CviBIII recognition sequence TCGA, and M · PstI, whose recognition sequence is CTGCAG. All of the N6-MTases contain the sequence Asp/Asn-Pro-Pro-Tyr (B-P-P-Y) referred to by Hattman et al. [J. Bacteriol. 164 (1985) 932-937] as region IV. The predicted secondary structure of this region forms a finger-like structure ('β finger') containing a β-pleated sheet (...XXXB), two β-turns (P-P) followed by another β-pleated sheet [Y/FXXX...].

Original languageEnglish (US)
Pages (from-to)253-259
Number of pages7
JournalGene
Volume74
Issue number1
DOIs
StatePublished - Dec 25 1988

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Methyltransferases
Adenine
Amino Acid Sequence
Nucleic Acids
DNA
Thermus
Viperidae
Viruses
Bacteria
Enzymes
Genes

Keywords

  • Chlorella virus
  • Recombinant DNA
  • Thermus aquaticus
  • restriction-modification

ASJC Scopus subject areas

  • Genetics

Cite this

The amino acid sequence of the eukaryotic DNA [N6-adenine]methyltransferase M·CviBIII, has regions of similarity with the prokaryotic isoschizomer M · TaqI and other DNA [N6-adenine] methyltransferases. / Narva, Kenneth E.; Van Etten, James L.; Slatko, Barton E.; Benner, Jack S.

In: Gene, Vol. 74, No. 1, 25.12.1988, p. 253-259.

Research output: Contribution to journalArticle

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abstract = "The sequences of the genes coding for M · CviBIII (from virus NC-1A which infects a eukaryotic alga) [Narva et al., Nucleic Acids Res. 15 (1987) 9807-9823] and M·TaqI (from the bacterium Thermus aquaticus) [Slatko et al., Nucleic Acids Res. 15 (1987) 9781-9796] have been determined recently. Both enzymes methylate adenine in the sequence TCGA. We have compared the predicted amino acid sequences of these two methyltransferases (MTases), with each other and with ten other N6A-MTases and find regions of similarity. M · CviBIII and M · TaqI were most closely related followed by M · PaeR7, whose recognition sequence (CTCGAG) contains the M . TaqI/M · CviBIII recognition sequence TCGA, and M · PstI, whose recognition sequence is CTGCAG. All of the N6-MTases contain the sequence Asp/Asn-Pro-Pro-Tyr (B-P-P-Y) referred to by Hattman et al. [J. Bacteriol. 164 (1985) 932-937] as region IV. The predicted secondary structure of this region forms a finger-like structure ('β finger') containing a β-pleated sheet (...XXXB), two β-turns (P-P) followed by another β-pleated sheet [Y/FXXX...].",
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