The addition of fenugreek extract (Trigonella foenum-graecum) to glucose feeding increases muscle glycogen resynthesis after exercise

Brent C. Ruby, S. E. Gaskill, Dustin R Slivka, S. G. Harger

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The purpose of this study was to determine the effects of ingesting an oral supplement containing 4-Hydroxyisoleucine (4-OH-Ile, isolated from fenugreek seeds [Trigonella foenum-graecum]) with a glucose beverage on rates of post-exercise muscle glycogen resynthesis in trained male cyclists. Following an overnight fast (12 hr), subjects completed a 90-minute glycogen depletion ride after which a muscle biopsy was obtained from the vastus lateralis. Immediately and 2 hours after the muscle biopsy, subjects ingested either an oral dose of dextrose (Glu) (1.8 g·kg BW-1) or 4-OH-Ile supplement (Glu+4-OH-Ile, including 2.0 mg·kg-1 4-OH-Ile with the same oral dose of dextrose) with a second muscle biopsy 4 hours after exercise. Post exercise muscle glycogen concentration was similar for both trials. Overall, there was a significant increase in glucose and insulin concentrations from time 0 throughout the majority of the 4-hour recovery period, with no significant differences between the two trials at any time point. Although muscle glycogen concentration significantly increased from immediately post exercise to 4 hr of recovery for both trials, the net rate of muscle glycogen resynthesis was 63% greater during Glu+4-OH-Ile (10.6±3.3 vs. 6.5±2.6 g·kg wet wt.-1·hr.-1 for the Glu+4-OH-Ile and Glu trials, respectively). These data demonstrate that when the fenugreek extract supplement (4-OH-Ile) is added to a high oral dose of dextrose, rates of post-exercise glycogen resynthesis are enhanced above dextrose alone.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalAmino Acids
Volume28
Issue number1
DOIs
StatePublished - Feb 1 2005

Fingerprint

Trigonella
Glycogen
Muscle
Glucose
Muscles
Biopsy
Recovery
Beverages
Quadriceps Muscle
hydroxide ion
Seed
Seeds
Insulin

Keywords

  • 4-OH-Isoleucine
  • Glucose uptake
  • Insulin
  • Muscle recovery
  • Post-exercise nutrition

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Endocrinology

Cite this

The addition of fenugreek extract (Trigonella foenum-graecum) to glucose feeding increases muscle glycogen resynthesis after exercise. / Ruby, Brent C.; Gaskill, S. E.; Slivka, Dustin R; Harger, S. G.

In: Amino Acids, Vol. 28, No. 1, 01.02.2005, p. 71-76.

Research output: Contribution to journalArticle

@article{4401f879782042b5b1a1fb322ca4580b,
title = "The addition of fenugreek extract (Trigonella foenum-graecum) to glucose feeding increases muscle glycogen resynthesis after exercise",
abstract = "The purpose of this study was to determine the effects of ingesting an oral supplement containing 4-Hydroxyisoleucine (4-OH-Ile, isolated from fenugreek seeds [Trigonella foenum-graecum]) with a glucose beverage on rates of post-exercise muscle glycogen resynthesis in trained male cyclists. Following an overnight fast (12 hr), subjects completed a 90-minute glycogen depletion ride after which a muscle biopsy was obtained from the vastus lateralis. Immediately and 2 hours after the muscle biopsy, subjects ingested either an oral dose of dextrose (Glu) (1.8 g·kg BW-1) or 4-OH-Ile supplement (Glu+4-OH-Ile, including 2.0 mg·kg-1 4-OH-Ile with the same oral dose of dextrose) with a second muscle biopsy 4 hours after exercise. Post exercise muscle glycogen concentration was similar for both trials. Overall, there was a significant increase in glucose and insulin concentrations from time 0 throughout the majority of the 4-hour recovery period, with no significant differences between the two trials at any time point. Although muscle glycogen concentration significantly increased from immediately post exercise to 4 hr of recovery for both trials, the net rate of muscle glycogen resynthesis was 63{\%} greater during Glu+4-OH-Ile (10.6±3.3 vs. 6.5±2.6 g·kg wet wt.-1·hr.-1 for the Glu+4-OH-Ile and Glu trials, respectively). These data demonstrate that when the fenugreek extract supplement (4-OH-Ile) is added to a high oral dose of dextrose, rates of post-exercise glycogen resynthesis are enhanced above dextrose alone.",
keywords = "4-OH-Isoleucine, Glucose uptake, Insulin, Muscle recovery, Post-exercise nutrition",
author = "Ruby, {Brent C.} and Gaskill, {S. E.} and Slivka, {Dustin R} and Harger, {S. G.}",
year = "2005",
month = "2",
day = "1",
doi = "10.1007/s00726-004-0143-z",
language = "English (US)",
volume = "28",
pages = "71--76",
journal = "Amino Acids",
issn = "0939-4451",
publisher = "Springer Wien",
number = "1",

}

TY - JOUR

T1 - The addition of fenugreek extract (Trigonella foenum-graecum) to glucose feeding increases muscle glycogen resynthesis after exercise

AU - Ruby, Brent C.

AU - Gaskill, S. E.

AU - Slivka, Dustin R

AU - Harger, S. G.

PY - 2005/2/1

Y1 - 2005/2/1

N2 - The purpose of this study was to determine the effects of ingesting an oral supplement containing 4-Hydroxyisoleucine (4-OH-Ile, isolated from fenugreek seeds [Trigonella foenum-graecum]) with a glucose beverage on rates of post-exercise muscle glycogen resynthesis in trained male cyclists. Following an overnight fast (12 hr), subjects completed a 90-minute glycogen depletion ride after which a muscle biopsy was obtained from the vastus lateralis. Immediately and 2 hours after the muscle biopsy, subjects ingested either an oral dose of dextrose (Glu) (1.8 g·kg BW-1) or 4-OH-Ile supplement (Glu+4-OH-Ile, including 2.0 mg·kg-1 4-OH-Ile with the same oral dose of dextrose) with a second muscle biopsy 4 hours after exercise. Post exercise muscle glycogen concentration was similar for both trials. Overall, there was a significant increase in glucose and insulin concentrations from time 0 throughout the majority of the 4-hour recovery period, with no significant differences between the two trials at any time point. Although muscle glycogen concentration significantly increased from immediately post exercise to 4 hr of recovery for both trials, the net rate of muscle glycogen resynthesis was 63% greater during Glu+4-OH-Ile (10.6±3.3 vs. 6.5±2.6 g·kg wet wt.-1·hr.-1 for the Glu+4-OH-Ile and Glu trials, respectively). These data demonstrate that when the fenugreek extract supplement (4-OH-Ile) is added to a high oral dose of dextrose, rates of post-exercise glycogen resynthesis are enhanced above dextrose alone.

AB - The purpose of this study was to determine the effects of ingesting an oral supplement containing 4-Hydroxyisoleucine (4-OH-Ile, isolated from fenugreek seeds [Trigonella foenum-graecum]) with a glucose beverage on rates of post-exercise muscle glycogen resynthesis in trained male cyclists. Following an overnight fast (12 hr), subjects completed a 90-minute glycogen depletion ride after which a muscle biopsy was obtained from the vastus lateralis. Immediately and 2 hours after the muscle biopsy, subjects ingested either an oral dose of dextrose (Glu) (1.8 g·kg BW-1) or 4-OH-Ile supplement (Glu+4-OH-Ile, including 2.0 mg·kg-1 4-OH-Ile with the same oral dose of dextrose) with a second muscle biopsy 4 hours after exercise. Post exercise muscle glycogen concentration was similar for both trials. Overall, there was a significant increase in glucose and insulin concentrations from time 0 throughout the majority of the 4-hour recovery period, with no significant differences between the two trials at any time point. Although muscle glycogen concentration significantly increased from immediately post exercise to 4 hr of recovery for both trials, the net rate of muscle glycogen resynthesis was 63% greater during Glu+4-OH-Ile (10.6±3.3 vs. 6.5±2.6 g·kg wet wt.-1·hr.-1 for the Glu+4-OH-Ile and Glu trials, respectively). These data demonstrate that when the fenugreek extract supplement (4-OH-Ile) is added to a high oral dose of dextrose, rates of post-exercise glycogen resynthesis are enhanced above dextrose alone.

KW - 4-OH-Isoleucine

KW - Glucose uptake

KW - Insulin

KW - Muscle recovery

KW - Post-exercise nutrition

UR - http://www.scopus.com/inward/record.url?scp=15244360956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15244360956&partnerID=8YFLogxK

U2 - 10.1007/s00726-004-0143-z

DO - 10.1007/s00726-004-0143-z

M3 - Article

VL - 28

SP - 71

EP - 76

JO - Amino Acids

JF - Amino Acids

SN - 0939-4451

IS - 1

ER -