The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica: Cloning, sequencing and homology analysis

Tran Tuan Hiep, Vladimir N. Kulikov, Vladimir N. Noskov, Genadij I. Sizonenko, Yuri O. Chernoff, Yuri I. Pavlov

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl‐5‐aminoimidazole‐carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed a single open reading frame, corresponding to a 543 amino acid sequence. The sequence of this putative protein has been compared to the proteins of homologous genes from S. cerevisiae, Schizosaccharomyces pombe, Escherichia coli, chicken and man. The analysis revealed remarkable homology between yeast AIRCs, while for other proteins homology was limited to defined regions.

Original languageEnglish (US)
Pages (from-to)1251-1258
Number of pages8
JournalYeast
Volume9
Issue number11
DOIs
StatePublished - Nov 1993

Fingerprint

Pichia
Cloning
Yeast
Organism Cloning
Genes
Yeasts
Saccharomyces cerevisiae
Proteins
Schizosaccharomyces
Open Reading Frames
Amino Acid Sequence
Chickens
Biosynthesis
Nucleotides
Escherichia coli
Amino acids
Mutation
DNA
Amino Acids

Keywords

  • Methylotrophic yeasts
  • gene homology analysis
  • phosphoribosyl‐5‐aminoimidazole‐carboxylase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica : Cloning, sequencing and homology analysis. / Hiep, Tran Tuan; Kulikov, Vladimir N.; Noskov, Vladimir N.; Sizonenko, Genadij I.; Chernoff, Yuri O.; Pavlov, Yuri I.

In: Yeast, Vol. 9, No. 11, 11.1993, p. 1251-1258.

Research output: Contribution to journalArticle

Hiep, Tran Tuan ; Kulikov, Vladimir N. ; Noskov, Vladimir N. ; Sizonenko, Genadij I. ; Chernoff, Yuri O. ; Pavlov, Yuri I. / The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica : Cloning, sequencing and homology analysis. In: Yeast. 1993 ; Vol. 9, No. 11. pp. 1251-1258.
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