The 1.8-Å resolution crystal structure of YDR533Cp from Saccharomyces cerevisiae: A member of the DJ-1/ThiJ/PfpI superfamily

Mark A. Wilson, Courtney V. St. Amour, Jennifer L. Collins, Dagmar Ringe, Gregory A. Petsko

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The yeast gene YDR533C encodes a protein belonging to the DJ-1/ThiJ/PfpI superfamily. This family includes the human protein DJ-1, which is mutated in autosomal recessive early-onset Parkinson's disease. The function of DJ-1 and its yeast homologue YDR533Cp is unknown. We report here the crystal structure of YDR533Cp at 1.8-Å resolution. The structure indicates that the closest relative to YDR533Cp is the Escherichia coli heat shock protein Hsp31 (YedU), which has both chaperone and protease activity. As expected, the overall fold of the core domain of YDR533Cp is also similar to that of DJ-1 and the bacterial protease PfpI. YDR533Cp contains a possible catalytic triad analogous to that of Hsp31 and an additional domain that is present in Hsp31 but is not seen in DJ-1 and other members of the family. The cysteine in this triad (Cys-138) is oxidized in this crystal structure, similar to modifications seen in the corresponding cysteine in the crystal structure of DJ-1. YDR533Cp appears to be a dimer both in solution and the crystal, but this dimer is formed by a different interface than that found in Hsp31 or other members of the superfamily.

Original languageEnglish (US)
Pages (from-to)1531-1536
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number6
DOIs
StatePublished - Feb 10 2004

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Cysteine
Saccharomyces cerevisiae
Peptide Hydrolases
Yeasts
Escherichia coli Proteins
Parkinsonian Disorders
Heat-Shock Proteins
Genes
Proteins
human PARK7 protein

ASJC Scopus subject areas

  • General

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The 1.8-Å resolution crystal structure of YDR533Cp from Saccharomyces cerevisiae : A member of the DJ-1/ThiJ/PfpI superfamily. / Wilson, Mark A.; St. Amour, Courtney V.; Collins, Jennifer L.; Ringe, Dagmar; Petsko, Gregory A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 6, 10.02.2004, p. 1531-1536.

Research output: Contribution to journalArticle

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