Tetracycline-inducible transfer of tetracycline resistance in Bacteroides fragilis in the absence of detectable plasmid DNA

A. Rashtchian, G. R. Dubes, S James Booth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tetracycline resistance of 3 B. fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. Tetracycline resistance markers could be transferred to another B. fragilis strain by filter mating. The transferability was inducible by subinhibitory concentrations of tetracycline and did not take pace in the absence of tetracycline. The optimum concentration of tetracycline for induction of transfer was about 2 μg/ml. The transfer was shown to be a conjugation-like process requiring cell-to-cell contact between donor and recipient. Screening of parental donor strains for the presence of plasmid DNA did not demonstrate any detectable plasmids in two of the strains. A 3.0 megadalton plasmid, designated pBY5, was present in the third donor strain. Mobilization of pBY5 by another plasmid (pBF4) showed that pBY5 did not carry the genes responsible for tetracycline resistance. It appears that the genes responsible for resistance to tetracycline as well as those responsible for conjugal transfer may be carried on the chromosome in all three donor strains.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalJournal of bacteriology
Volume150
Issue number1
StatePublished - Jan 1 1982

Fingerprint

Tetracycline Resistance
Bacteroides fragilis
Tetracycline
Plasmids
DNA
Donor Selection
Genes
Chromosomes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Tetracycline-inducible transfer of tetracycline resistance in Bacteroides fragilis in the absence of detectable plasmid DNA. / Rashtchian, A.; Dubes, G. R.; Booth, S James.

In: Journal of bacteriology, Vol. 150, No. 1, 01.01.1982, p. 141-147.

Research output: Contribution to journalArticle

@article{f6e3ddfb1694451098c125d53b193d29,
title = "Tetracycline-inducible transfer of tetracycline resistance in Bacteroides fragilis in the absence of detectable plasmid DNA",
abstract = "Tetracycline resistance of 3 B. fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. Tetracycline resistance markers could be transferred to another B. fragilis strain by filter mating. The transferability was inducible by subinhibitory concentrations of tetracycline and did not take pace in the absence of tetracycline. The optimum concentration of tetracycline for induction of transfer was about 2 μg/ml. The transfer was shown to be a conjugation-like process requiring cell-to-cell contact between donor and recipient. Screening of parental donor strains for the presence of plasmid DNA did not demonstrate any detectable plasmids in two of the strains. A 3.0 megadalton plasmid, designated pBY5, was present in the third donor strain. Mobilization of pBY5 by another plasmid (pBF4) showed that pBY5 did not carry the genes responsible for tetracycline resistance. It appears that the genes responsible for resistance to tetracycline as well as those responsible for conjugal transfer may be carried on the chromosome in all three donor strains.",
author = "A. Rashtchian and Dubes, {G. R.} and Booth, {S James}",
year = "1982",
month = "1",
day = "1",
language = "English (US)",
volume = "150",
pages = "141--147",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Tetracycline-inducible transfer of tetracycline resistance in Bacteroides fragilis in the absence of detectable plasmid DNA

AU - Rashtchian, A.

AU - Dubes, G. R.

AU - Booth, S James

PY - 1982/1/1

Y1 - 1982/1/1

N2 - Tetracycline resistance of 3 B. fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. Tetracycline resistance markers could be transferred to another B. fragilis strain by filter mating. The transferability was inducible by subinhibitory concentrations of tetracycline and did not take pace in the absence of tetracycline. The optimum concentration of tetracycline for induction of transfer was about 2 μg/ml. The transfer was shown to be a conjugation-like process requiring cell-to-cell contact between donor and recipient. Screening of parental donor strains for the presence of plasmid DNA did not demonstrate any detectable plasmids in two of the strains. A 3.0 megadalton plasmid, designated pBY5, was present in the third donor strain. Mobilization of pBY5 by another plasmid (pBF4) showed that pBY5 did not carry the genes responsible for tetracycline resistance. It appears that the genes responsible for resistance to tetracycline as well as those responsible for conjugal transfer may be carried on the chromosome in all three donor strains.

AB - Tetracycline resistance of 3 B. fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. Tetracycline resistance markers could be transferred to another B. fragilis strain by filter mating. The transferability was inducible by subinhibitory concentrations of tetracycline and did not take pace in the absence of tetracycline. The optimum concentration of tetracycline for induction of transfer was about 2 μg/ml. The transfer was shown to be a conjugation-like process requiring cell-to-cell contact between donor and recipient. Screening of parental donor strains for the presence of plasmid DNA did not demonstrate any detectable plasmids in two of the strains. A 3.0 megadalton plasmid, designated pBY5, was present in the third donor strain. Mobilization of pBY5 by another plasmid (pBF4) showed that pBY5 did not carry the genes responsible for tetracycline resistance. It appears that the genes responsible for resistance to tetracycline as well as those responsible for conjugal transfer may be carried on the chromosome in all three donor strains.

UR - http://www.scopus.com/inward/record.url?scp=0020052365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020052365&partnerID=8YFLogxK

M3 - Article

VL - 150

SP - 141

EP - 147

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 1

ER -