Testosterone meets albumin-the molecular mechanism of sex hormone transport by serum albumins

Mateusz P. Czub, Barat S. Venkataramany, Karolina A. Majorek, Katarzyna B. Handing, Przemyslaw J. Porebski, Sandya R. Beeram, Kyungah Suh, Ashley G. Woolfork, David S Hage, Ivan G. Shabalin, Wladek Minor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Serum albumin is the most abundant protein in mammalian blood plasma and is responsible for the transport of metals, drugs, and various metabolites, including hormones. We report the first albumin structure in complex with testosterone, the primary male sex hormone. Testosterone is bound in two sites, neither of which overlaps with the previously suggested Sudlow site I. We determined the binding constant of testosterone to equine and human albumins by two different methods: tryptophan fluorescence quenching and ultrafast affinity extraction. The binding studies and similarities between residues comprising the binding sites on serum albumins suggest that testosterone binds to the same sites on both proteins. Our comparative analysis of albumin complexes with hormones, drugs, and other biologically relevant compounds strongly suggests interference between a number of compounds present in blood and testosterone transport by serum albumin. We discuss a possible link between our findings and some phenomena observed in human patients, such as low testosterone levels in diabetic patients.

Original languageEnglish (US)
Pages (from-to)1607-1618
Number of pages12
JournalChemical Science
Volume10
Issue number6
DOIs
StatePublished - Jan 1 2019

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Gonadal Steroid Hormones
Serum Albumin
Testosterone
Albumins
Blood
Hormones
Metabolites
Tryptophan
Pharmaceutical Preparations
Quenching
Proteins
Metals
Fluorescence
Binding Sites
Plasmas

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Czub, M. P., Venkataramany, B. S., Majorek, K. A., Handing, K. B., Porebski, P. J., Beeram, S. R., ... Minor, W. (2019). Testosterone meets albumin-the molecular mechanism of sex hormone transport by serum albumins. Chemical Science, 10(6), 1607-1618. https://doi.org/10.1039/c8sc04397c

Testosterone meets albumin-the molecular mechanism of sex hormone transport by serum albumins. / Czub, Mateusz P.; Venkataramany, Barat S.; Majorek, Karolina A.; Handing, Katarzyna B.; Porebski, Przemyslaw J.; Beeram, Sandya R.; Suh, Kyungah; Woolfork, Ashley G.; Hage, David S; Shabalin, Ivan G.; Minor, Wladek.

In: Chemical Science, Vol. 10, No. 6, 01.01.2019, p. 1607-1618.

Research output: Contribution to journalArticle

Czub, MP, Venkataramany, BS, Majorek, KA, Handing, KB, Porebski, PJ, Beeram, SR, Suh, K, Woolfork, AG, Hage, DS, Shabalin, IG & Minor, W 2019, 'Testosterone meets albumin-the molecular mechanism of sex hormone transport by serum albumins', Chemical Science, vol. 10, no. 6, pp. 1607-1618. https://doi.org/10.1039/c8sc04397c
Czub MP, Venkataramany BS, Majorek KA, Handing KB, Porebski PJ, Beeram SR et al. Testosterone meets albumin-the molecular mechanism of sex hormone transport by serum albumins. Chemical Science. 2019 Jan 1;10(6):1607-1618. https://doi.org/10.1039/c8sc04397c
Czub, Mateusz P. ; Venkataramany, Barat S. ; Majorek, Karolina A. ; Handing, Katarzyna B. ; Porebski, Przemyslaw J. ; Beeram, Sandya R. ; Suh, Kyungah ; Woolfork, Ashley G. ; Hage, David S ; Shabalin, Ivan G. ; Minor, Wladek. / Testosterone meets albumin-the molecular mechanism of sex hormone transport by serum albumins. In: Chemical Science. 2019 ; Vol. 10, No. 6. pp. 1607-1618.
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