Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice

C. M. Schmidt, Lin Chen Hua Lin Chen, I. Chiu, R. G. Ehlenfeldt, J. S. Hunt, H. T. Orr

Research output: Contribution to journalArticle

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Abstract

HLA-G, a nonclassical class I molecule, is expressed by trophoblasts, the only fetal cells in direct contact with maternal tissue. Results of previous experiments suggested that a 244-bp region located over 1 kb 5' from exon 1 is critical for extraembryonic expression of HLA-G in transgenic mice. We report here the production of HLA-G transgenic lines with a 6.0-kb HLA-G transgene that includes the 244-bp region. These lines exhibit copy number- dependent and developmentally appropriate transgene expression. One HLA-G transgenic line, G.3.2, exhibits extraembryonic HLA-G mRNA expression levels similar to those seen in human extraembryonic tissues. Studies of the cell- type-specific localization of HLA-G mRNA in placentas from the G.3.2 HLA-G transgenic mouse line show predominant localization of the HLA-G message in the spongiotrophoblast layer. This layer is in a similar anatomic location to the HLA-G-expressing human cytotrophoblast shell. The G.3.2 HLA-G transgenic mouse line should serve as an appropriate model for the study of HLA-G function at the maternal-fetal interface.

Original languageEnglish (US)
Pages (from-to)619-629
Number of pages11
JournalJournal of Immunology
Volume155
Issue number2
StatePublished - Jan 1 1995

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HLA-G Antigens
Transgenic Mice
Messenger RNA
Trophoblasts
Transgenes
Mothers
Placenta

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Schmidt, C. M., Hua Lin Chen, L. C., Chiu, I., Ehlenfeldt, R. G., Hunt, J. S., & Orr, H. T. (1995). Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice. Journal of Immunology, 155(2), 619-629.

Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice. / Schmidt, C. M.; Hua Lin Chen, Lin Chen; Chiu, I.; Ehlenfeldt, R. G.; Hunt, J. S.; Orr, H. T.

In: Journal of Immunology, Vol. 155, No. 2, 01.01.1995, p. 619-629.

Research output: Contribution to journalArticle

Schmidt, CM, Hua Lin Chen, LC, Chiu, I, Ehlenfeldt, RG, Hunt, JS & Orr, HT 1995, 'Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice', Journal of Immunology, vol. 155, no. 2, pp. 619-629.
Schmidt CM, Hua Lin Chen LC, Chiu I, Ehlenfeldt RG, Hunt JS, Orr HT. Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice. Journal of Immunology. 1995 Jan 1;155(2):619-629.
Schmidt, C. M. ; Hua Lin Chen, Lin Chen ; Chiu, I. ; Ehlenfeldt, R. G. ; Hunt, J. S. ; Orr, H. T. / Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice. In: Journal of Immunology. 1995 ; Vol. 155, No. 2. pp. 619-629.
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