Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice

C. M. Schmidt, Lin Chen Hua Lin Chen, I. Chiu, R. G. Ehlenfeldt, J. S. Hunt, H. T. Orr

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Abstract

HLA-G, a nonclassical class I molecule, is expressed by trophoblasts, the only fetal cells in direct contact with maternal tissue. Results of previous experiments suggested that a 244-bp region located over 1 kb 5' from exon 1 is critical for extraembryonic expression of HLA-G in transgenic mice. We report here the production of HLA-G transgenic lines with a 6.0-kb HLA-G transgene that includes the 244-bp region. These lines exhibit copy number- dependent and developmentally appropriate transgene expression. One HLA-G transgenic line, G.3.2, exhibits extraembryonic HLA-G mRNA expression levels similar to those seen in human extraembryonic tissues. Studies of the cell- type-specific localization of HLA-G mRNA in placentas from the G.3.2 HLA-G transgenic mouse line show predominant localization of the HLA-G message in the spongiotrophoblast layer. This layer is in a similar anatomic location to the HLA-G-expressing human cytotrophoblast shell. The G.3.2 HLA-G transgenic mouse line should serve as an appropriate model for the study of HLA-G function at the maternal-fetal interface.

Original languageEnglish (US)
Pages (from-to)619-629
Number of pages11
JournalJournal of Immunology
Volume155
Issue number2
Publication statusPublished - Jan 1 1995

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Schmidt, C. M., Hua Lin Chen, L. C., Chiu, I., Ehlenfeldt, R. G., Hunt, J. S., & Orr, H. T. (1995). Temporal and spatial expression of HLA-G messenger RNA in extraembryonic tissues of transgenic mice. Journal of Immunology, 155(2), 619-629.