Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide)

Harender S. Bisht, Devika Soundara Manickam, Yezi You, David Oupicky

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

End-functionalized poly(N-isopropylacrylamide) (PNIPA) was synthesized by living free radical polymerization and conventional free radical polymerization and was used to prepare graft copolymers with poly(ethylenimine) (PEI). The copolymers exhibited lower critical solution temperature (LCST) behavior between 30 and 32 °C and formed complexes with plasmid DNA. The LCST of the copolymers in the DNA complexes increased slightly to ∼34-35 °C. Cytotoxicity of the copolymers was evaluated by measuring lactate dehydrogenase (LDH) release from cells. The copolymers exhibited temperature-dependent toxicity, with higher levels of LDH release observed at temperatures above the LCST. Cellular uptake and transfection activity of the DNA complexes with the PEI-g-PNIPA copolymers were lower than those of the control PEI/DNA complexes at temperature below the LCST but increased to the PEI/DNA levels at temperatures above the LCST.

Original languageEnglish (US)
Pages (from-to)1169-1178
Number of pages10
JournalBiomacromolecules
Volume7
Issue number4
DOIs
StatePublished - Apr 1 2006

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Grafts
DNA
Transplants
Temperature
Polyetherimides
Copolymers
Free radical polymerization
L-Lactate Dehydrogenase
Polymerization
Free Radicals
poly-N-isopropylacrylamide
Graft copolymers
Cytotoxicity
Transfection
Toxicity
Plasmids
Thermodynamic properties

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide). / Bisht, Harender S.; Manickam, Devika Soundara; You, Yezi; Oupicky, David.

In: Biomacromolecules, Vol. 7, No. 4, 01.04.2006, p. 1169-1178.

Research output: Contribution to journalArticle

Bisht, Harender S. ; Manickam, Devika Soundara ; You, Yezi ; Oupicky, David. / Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide). In: Biomacromolecules. 2006 ; Vol. 7, No. 4. pp. 1169-1178.
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