Technical note: Comparison of salivary and serum cortisol concentrations after adrenocorticotropic hormone challenge in ewes

D. T. Yates, T. T. Ross, D. M. Hallford, L. J. Yates, R. L. Wesley

Research output: Contribution to journalArticle

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Abstract

An ACTH challenge was conducted to determine if salivary cortisol concentration reflects serum cortisol concentration in ewes. Twelve yearling ewes (64.0 ± 1.2 kg) were administered ACTH (100 IU, intravenously) or saline. Serum and salivary samples were collected at 30-min intervals for 2 h before ACTH administration, at 15-min intervals for 2 h after treatment, and at 30-min intervals for an additional 3 h, and cortisol concentration was determined by RIA. Although ewes responded to ACTH and saline, cortisol concentration was greater (P < 0.001) in ACTH-treated ewes from 15 to 120 min and tended to be greater (P = 0.054) at 150 min after challenge in serum. In saliva, cortisol concentration was greater (P < 0.001) in ACTH-treated ewes from 30 to 120 min and tended to be greater (P = 0.092) at 15 min after challenge. No difference was observed between ACTH-treated ewes and controls for time to peak serum cortisol concentration (P = 0.126) and time to peak salivary cortisol concentration (P = 0.109), or between saliva and serum for time to peak cortisol concentration (P = 0.220) and return to baseline cortisol concentration (P = 0.341). The serum (P = 0.009) and salivary (P = 0.050) cortisol areas under the curve between 0 and 150 min were greater for ACTH-treated ewes than controls, and serum (P = 0.002) and salivary (P < 0.001) cortisol return to baseline concentration was longer for ACTH-treated ewes. The correlation coefficient between serum and salivary cortisol concentrations was 0.88 (P < 0.001). These data indicate that salivary cortisol concentration is closely related to serum cortisol concentration and that the former may represent a suitable noninvasive alternative to blood collection for measurement of cortisol in sheep.

Original languageEnglish (US)
Pages (from-to)599-603
Number of pages5
JournalJournal of animal science
Volume88
Issue number2
DOIs
StatePublished - Feb 1 2010

Fingerprint

corticotropin
Adrenocorticotropic Hormone
cortisol
Hydrocortisone
ewes
Serum
blood serum
saliva
Saliva
yearlings
Area Under Curve
Sheep

Keywords

  • Adrenocorticotropic hormone challenge
  • Saliva collection
  • Salivary cortisol

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Technical note : Comparison of salivary and serum cortisol concentrations after adrenocorticotropic hormone challenge in ewes. / Yates, D. T.; Ross, T. T.; Hallford, D. M.; Yates, L. J.; Wesley, R. L.

In: Journal of animal science, Vol. 88, No. 2, 01.02.2010, p. 599-603.

Research output: Contribution to journalArticle

Yates, D. T. ; Ross, T. T. ; Hallford, D. M. ; Yates, L. J. ; Wesley, R. L. / Technical note : Comparison of salivary and serum cortisol concentrations after adrenocorticotropic hormone challenge in ewes. In: Journal of animal science. 2010 ; Vol. 88, No. 2. pp. 599-603.
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