TCR usage and functional capabilities of human γδ T cells at birth

Craig T. Morita, Christina M. Parker, Michael B. Brenner, Hamid Band

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Little is known about the γδ T cells in human neonatal umbilical cord blood. To compare neonatal cord blood and adult blood γδ T cells, we studied the Vγ and Vδ gene segment usage in these populations by flow cytometry, and we derived cord blood γδ T cell clones to determine their functional capabilities. Unlike adult blood γδ T cells that predominantly express Vγ2Vδ2 TCRs, neonatal cord blood γδ T cells expressed diverse Vγ and Vδ gene segments paired in a variety of combinations rarely observed in adults. γδ T cells clones derived from neonatal cord blood similarly expressed a diverse array of TCRs. These cord blood-derived γδ T cells clones had weak cytolytic activity when assayed for K562 tumor cell killing, lectin-mediated cytolysis, and redirected cytolysis. They also expressed lower levels of the CD2, LFA-1, and CD45RO cell surface receptors as compared with strongly cytolytic adult γδ T cell clones. These properties of the cord blood-derived γδ T cells clones, weak cytotoxic activity and low adhesion molecule expression, were similar to the properties of the CD4+ subset of adult γδ T cells. Thus, neonatal γδ T cells are functionally different from the majority of adult γδ T cells and display a distinct TCR repertoire and accessory molecule profile.

Original languageEnglish (US)
Pages (from-to)3979-3988
Number of pages10
JournalJournal of Immunology
Volume153
Issue number9
StatePublished - Nov 1 1994

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Parturition
T-Lymphocytes
Fetal Blood
Clone Cells
Blood Cells
Lymphocyte Function-Associated Antigen-1
K562 Cells
Cell Surface Receptors
T-Lymphocyte Subsets
Lectins
Genes
Flow Cytometry
Population
Neoplasms

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Morita, C. T., Parker, C. M., Brenner, M. B., & Band, H. (1994). TCR usage and functional capabilities of human γδ T cells at birth. Journal of Immunology, 153(9), 3979-3988.

TCR usage and functional capabilities of human γδ T cells at birth. / Morita, Craig T.; Parker, Christina M.; Brenner, Michael B.; Band, Hamid.

In: Journal of Immunology, Vol. 153, No. 9, 01.11.1994, p. 3979-3988.

Research output: Contribution to journalArticle

Morita, CT, Parker, CM, Brenner, MB & Band, H 1994, 'TCR usage and functional capabilities of human γδ T cells at birth', Journal of Immunology, vol. 153, no. 9, pp. 3979-3988.
Morita CT, Parker CM, Brenner MB, Band H. TCR usage and functional capabilities of human γδ T cells at birth. Journal of Immunology. 1994 Nov 1;153(9):3979-3988.
Morita, Craig T. ; Parker, Christina M. ; Brenner, Michael B. ; Band, Hamid. / TCR usage and functional capabilities of human γδ T cells at birth. In: Journal of Immunology. 1994 ; Vol. 153, No. 9. pp. 3979-3988.
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