TATA-binding protein recognition and bending of a consensus promoter are protein species dependent

JoDell E. Whittington, Roberto F. Delgadillo, Torrissa J. Attebury, Laura K. Parkhurst, Margaret A. Daugherty, Lawrence J Parkhurst

Research output: Contribution to journalArticle

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Abstract

The structure and behavior of full-length human TBP binding the adenovirus major late promoter (AdMLP) have been characterized using biophysical methods. The human protein induces a 97° bend in DNAAdMLP. The high-resolution functional data provide a quantitative energetic and kinetic description of the partial reaction sequence as native human TBP binds rapidly to a consensus promoter with high affinity. The reaction proceeds with successive formation of three bound species, all having strongly bent DNA, with the concurrence of binding and bending demonstrated by both fluorescence and anisotropy stopped flow. These results establish the protein species dependence of the TBP-DNAAdMLP structure and recognition mechanism. Additionally, the strong correlation between the DNA bend angle and transcription efficiency demonstrated previously for yeast TBP is shown to extend to human TBP. The heterologous NH2-terminal domains are the apparent source of the species-specific differences. Together with previous studies the present work establishes that TBPwt-DNATATA function and structure depend both on the TATA box sequence and on the TBP species.

Original languageEnglish (US)
Pages (from-to)7264-7273
Number of pages10
JournalBiochemistry
Volume47
Issue number27
DOIs
StatePublished - Jul 8 2008

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TATA-Box Binding Protein
Consensus
Proteins
TATA Box
Fluorescence Polarization
DNA
Transcription
Population Groups
Adenoviridae
Yeast
Recognition (Psychology)
tributyl phosphate
Anisotropy
Yeasts
Fluorescence
Efficiency
Kinetics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Whittington, J. E., Delgadillo, R. F., Attebury, T. J., Parkhurst, L. K., Daugherty, M. A., & Parkhurst, L. J. (2008). TATA-binding protein recognition and bending of a consensus promoter are protein species dependent. Biochemistry, 47(27), 7264-7273. https://doi.org/10.1021/bi800139w

TATA-binding protein recognition and bending of a consensus promoter are protein species dependent. / Whittington, JoDell E.; Delgadillo, Roberto F.; Attebury, Torrissa J.; Parkhurst, Laura K.; Daugherty, Margaret A.; Parkhurst, Lawrence J.

In: Biochemistry, Vol. 47, No. 27, 08.07.2008, p. 7264-7273.

Research output: Contribution to journalArticle

Whittington, JE, Delgadillo, RF, Attebury, TJ, Parkhurst, LK, Daugherty, MA & Parkhurst, LJ 2008, 'TATA-binding protein recognition and bending of a consensus promoter are protein species dependent', Biochemistry, vol. 47, no. 27, pp. 7264-7273. https://doi.org/10.1021/bi800139w
Whittington JE, Delgadillo RF, Attebury TJ, Parkhurst LK, Daugherty MA, Parkhurst LJ. TATA-binding protein recognition and bending of a consensus promoter are protein species dependent. Biochemistry. 2008 Jul 8;47(27):7264-7273. https://doi.org/10.1021/bi800139w
Whittington, JoDell E. ; Delgadillo, Roberto F. ; Attebury, Torrissa J. ; Parkhurst, Laura K. ; Daugherty, Margaret A. ; Parkhurst, Lawrence J. / TATA-binding protein recognition and bending of a consensus promoter are protein species dependent. In: Biochemistry. 2008 ; Vol. 47, No. 27. pp. 7264-7273.
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