Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases

Manabu Furukawa, Yizhou Joseph He, Christoph Borchers, Yue Xiong

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

The concentrations and functions of many cellular proteins are regulated by the ubiquitin pathway. Cullin family proteins bind with the RING-finger protein Roc1 to recruit the ubiquitin-conjugating enzyme (E2) to the ubiquitin ligase complex (E3). Cul1 and Cul7, but not other cullins, bind to an adaptor protein, Skp1. Cul1 associates with one of many F-box proteins through Skp1 to assemble various SCF-Roc1 E3 ligases that each selectively ubiquitinate one or more specific substrates. Here, we show that Cul3, but not other cullins, binds directly to multiple BTB domains through a conserved amino-terminal domain. In vitro, Cul3 promoted ubiquitination of Caenorhabditis elegans MEI-1, a katanin-like protein whose degradation requires the function of both Cul3 and BTB protein MEL-26. We suggest that in vivo there exists a potentially large number of BCR3 (BTB-Cul3-Roc1) E3 ubiquitin ligases.

Original languageEnglish (US)
Pages (from-to)1001-1007
Number of pages7
JournalNature Cell Biology
Volume5
Issue number11
DOIs
StatePublished - Nov 1 2003

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Cullin Proteins
Ubiquitin-Protein Ligases
Ubiquitination
Protein Transport
Ligases
Ubiquitin
S-Phase Kinase-Associated Proteins
F-Box Proteins
Ubiquitin-Conjugating Enzymes
Proteins
Caenorhabditis elegans
Proteolysis
Fingers

ASJC Scopus subject areas

  • Cell Biology

Cite this

Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. / Furukawa, Manabu; He, Yizhou Joseph; Borchers, Christoph; Xiong, Yue.

In: Nature Cell Biology, Vol. 5, No. 11, 01.11.2003, p. 1001-1007.

Research output: Contribution to journalArticle

Furukawa, M, He, YJ, Borchers, C & Xiong, Y 2003, 'Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases', Nature Cell Biology, vol. 5, no. 11, pp. 1001-1007. https://doi.org/10.1038/ncb1056
Furukawa, Manabu ; He, Yizhou Joseph ; Borchers, Christoph ; Xiong, Yue. / Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. In: Nature Cell Biology. 2003 ; Vol. 5, No. 11. pp. 1001-1007.
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