Targeted Gene Delivery with a Low Molecular Weight Glycopeptide Carrier

Manpreet S. Wadhwa, Kevin G. Rice, Daren L Knoell, Anthony P. Young

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

A low molecular weight glycopeptide carrier was prepared by coupling a tyrosinamide-triantennary oligosaccharide to dp19 poly-L-lysine resulting in a 1:1 conjugate. The glycopeptide carrier complexed with plasmid DNA as evidenced by displacement of intercalated dye, light scattering by condensed DNA, and immobility of complexed DNA upon agarose gel electrophoresis. DNA-carrier complexes were endocytosed into HepG2 cells via the asialoglycoprotein receptor due to recognition of terminal galactose residues on the oligosaccharide. The resulting luciferase reporter gene expression was dramatically influenced by the solubility of complexes, the extent of complexation, and the presence of the lysosomotropic agent chloroquine. The results suggest that low molecular weight glycopeptides may be suitable for further development as well-defined DNA carriers for receptor-mediated gene delivery in vivo.

Original languageEnglish (US)
Pages (from-to)283-291
Number of pages9
JournalBioconjugate Chemistry
Volume6
Issue number3
DOIs
StatePublished - Jan 1 1995

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Glycopeptides
DNA
Genes
Molecular Weight
Molecular weight
Oligosaccharides
Asialoglycoprotein Receptor
Agar Gel Electrophoresis
Chloroquine
Hep G2 Cells
Endocytosis
Luciferases
Galactose
Reporter Genes
Solubility
Lysine
Electrophoresis
Complexation
Plasmids
Coloring Agents

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Targeted Gene Delivery with a Low Molecular Weight Glycopeptide Carrier. / Wadhwa, Manpreet S.; Rice, Kevin G.; Knoell, Daren L; Young, Anthony P.

In: Bioconjugate Chemistry, Vol. 6, No. 3, 01.01.1995, p. 283-291.

Research output: Contribution to journalArticle

Wadhwa, Manpreet S. ; Rice, Kevin G. ; Knoell, Daren L ; Young, Anthony P. / Targeted Gene Delivery with a Low Molecular Weight Glycopeptide Carrier. In: Bioconjugate Chemistry. 1995 ; Vol. 6, No. 3. pp. 283-291.
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