Targeted antimicrobial activity of a specific IgG-SMAP28 conjugate against Porphyromonas gingivalis in a mixed culture

Michael R. Franzman, Kindra K. Burnell, Farideh H. Dehkordi-Vakil, Janet M. Guthmiller, Deborah V. Dawson, Kim A. Brogden

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. To assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (SMAP) 28 was linked to affinity- and protein G-purified rabbit immunoglobulin G (IgG) antibodies specific to the outer surface of Porphyromonas gingivalis strain 381. The selective activity of the P. gingivalis IgG-SMAP28 conjugate was then assessed by adding it to an artificially generated microbial community containing P. gingivalis, Aggregatibacter actinomycetemcomitans and Peptostreptococcus micros. The specificity of the P. gingivalis IgG-SMAP28 conjugate in this mixed culture was concentration-dependent. The conjugate at 50 μg protein/mL lacked specificity and killed P. gingivalis, A. actinomycetemcomitans and P. micros. The conjugate at 20 μg protein/mL was more specific and killed P. gingivalis. This is an initial step to develop a selective antimicrobial agent that can eliminate a specific periodontal pathogen, such as P. gingivalis, from patients with periodontal disease without harming the normal commensal flora.

Original languageEnglish (US)
Pages (from-to)14-20
Number of pages7
JournalInternational Journal of Antimicrobial Agents
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Porphyromonas gingivalis
Immunoglobulin G
Aggregatibacter actinomycetemcomitans
Peptostreptococcus
Peptides
Proteins
Antibodies
Periodontal Diseases
Anti-Infective Agents
Sheep
Anti-Bacterial Agents
Rabbits
Ligands
Bacteria
Pharmaceutical Preparations

Keywords

  • Aggregatibacter actinomycetemcomitans
  • Cathelicidins
  • Peptostreptococcus micros
  • Porphyromonas gingivalis
  • SMAP28
  • Targeted antimicrobial activity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Targeted antimicrobial activity of a specific IgG-SMAP28 conjugate against Porphyromonas gingivalis in a mixed culture. / Franzman, Michael R.; Burnell, Kindra K.; Dehkordi-Vakil, Farideh H.; Guthmiller, Janet M.; Dawson, Deborah V.; Brogden, Kim A.

In: International Journal of Antimicrobial Agents, Vol. 33, No. 1, 01.01.2009, p. 14-20.

Research output: Contribution to journalArticle

Franzman, Michael R. ; Burnell, Kindra K. ; Dehkordi-Vakil, Farideh H. ; Guthmiller, Janet M. ; Dawson, Deborah V. ; Brogden, Kim A. / Targeted antimicrobial activity of a specific IgG-SMAP28 conjugate against Porphyromonas gingivalis in a mixed culture. In: International Journal of Antimicrobial Agents. 2009 ; Vol. 33, No. 1. pp. 14-20.
@article{d7db41497def42caa05a37ef83feb46d,
title = "Targeted antimicrobial activity of a specific IgG-SMAP28 conjugate against Porphyromonas gingivalis in a mixed culture",
abstract = "Antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. To assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (SMAP) 28 was linked to affinity- and protein G-purified rabbit immunoglobulin G (IgG) antibodies specific to the outer surface of Porphyromonas gingivalis strain 381. The selective activity of the P. gingivalis IgG-SMAP28 conjugate was then assessed by adding it to an artificially generated microbial community containing P. gingivalis, Aggregatibacter actinomycetemcomitans and Peptostreptococcus micros. The specificity of the P. gingivalis IgG-SMAP28 conjugate in this mixed culture was concentration-dependent. The conjugate at 50 μg protein/mL lacked specificity and killed P. gingivalis, A. actinomycetemcomitans and P. micros. The conjugate at 20 μg protein/mL was more specific and killed P. gingivalis. This is an initial step to develop a selective antimicrobial agent that can eliminate a specific periodontal pathogen, such as P. gingivalis, from patients with periodontal disease without harming the normal commensal flora.",
keywords = "Aggregatibacter actinomycetemcomitans, Cathelicidins, Peptostreptococcus micros, Porphyromonas gingivalis, SMAP28, Targeted antimicrobial activity",
author = "Franzman, {Michael R.} and Burnell, {Kindra K.} and Dehkordi-Vakil, {Farideh H.} and Guthmiller, {Janet M.} and Dawson, {Deborah V.} and Brogden, {Kim A.}",
year = "2009",
month = "1",
day = "1",
doi = "10.1016/j.ijantimicag.2008.05.021",
language = "English (US)",
volume = "33",
pages = "14--20",
journal = "International Journal of Antimicrobial Agents",
issn = "0924-8579",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Targeted antimicrobial activity of a specific IgG-SMAP28 conjugate against Porphyromonas gingivalis in a mixed culture

AU - Franzman, Michael R.

AU - Burnell, Kindra K.

AU - Dehkordi-Vakil, Farideh H.

AU - Guthmiller, Janet M.

AU - Dawson, Deborah V.

AU - Brogden, Kim A.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. To assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (SMAP) 28 was linked to affinity- and protein G-purified rabbit immunoglobulin G (IgG) antibodies specific to the outer surface of Porphyromonas gingivalis strain 381. The selective activity of the P. gingivalis IgG-SMAP28 conjugate was then assessed by adding it to an artificially generated microbial community containing P. gingivalis, Aggregatibacter actinomycetemcomitans and Peptostreptococcus micros. The specificity of the P. gingivalis IgG-SMAP28 conjugate in this mixed culture was concentration-dependent. The conjugate at 50 μg protein/mL lacked specificity and killed P. gingivalis, A. actinomycetemcomitans and P. micros. The conjugate at 20 μg protein/mL was more specific and killed P. gingivalis. This is an initial step to develop a selective antimicrobial agent that can eliminate a specific periodontal pathogen, such as P. gingivalis, from patients with periodontal disease without harming the normal commensal flora.

AB - Antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. To assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (SMAP) 28 was linked to affinity- and protein G-purified rabbit immunoglobulin G (IgG) antibodies specific to the outer surface of Porphyromonas gingivalis strain 381. The selective activity of the P. gingivalis IgG-SMAP28 conjugate was then assessed by adding it to an artificially generated microbial community containing P. gingivalis, Aggregatibacter actinomycetemcomitans and Peptostreptococcus micros. The specificity of the P. gingivalis IgG-SMAP28 conjugate in this mixed culture was concentration-dependent. The conjugate at 50 μg protein/mL lacked specificity and killed P. gingivalis, A. actinomycetemcomitans and P. micros. The conjugate at 20 μg protein/mL was more specific and killed P. gingivalis. This is an initial step to develop a selective antimicrobial agent that can eliminate a specific periodontal pathogen, such as P. gingivalis, from patients with periodontal disease without harming the normal commensal flora.

KW - Aggregatibacter actinomycetemcomitans

KW - Cathelicidins

KW - Peptostreptococcus micros

KW - Porphyromonas gingivalis

KW - SMAP28

KW - Targeted antimicrobial activity

UR - http://www.scopus.com/inward/record.url?scp=59349093299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59349093299&partnerID=8YFLogxK

U2 - 10.1016/j.ijantimicag.2008.05.021

DO - 10.1016/j.ijantimicag.2008.05.021

M3 - Article

C2 - 18778918

AN - SCOPUS:59349093299

VL - 33

SP - 14

EP - 20

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 1

ER -