Tapasin decreases immune responsiveness to a model tumor antigen

Heth R. Turnquist, Karl G. Kohlgraf, Mary M. McIlhaney, R Lee Mosley, Michael A Hollingsworth, Joyce C Solheim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The T-cell response against cancer is dependent on the cell surface presentation of tumor-associated or tumor-specific peptides by major histocompatibility complex (MHC) class I molecules. We found that tapasin, a chaperone protein that normally assists in the assembly of MHC class I molecules, is undetectable in an unstimulated pancreatic tumor cell line, Panc02, and only very weakly expressed after γ-interferon stimulation. Transfection of tapasin into the Panc02 cells did not quantitatively increase MHC class I surface expression or detectably affect MHC class I association with peptide and β2-microglubulin (β2m). However, we found that transfected tapasin downregulated immune reactivity against a model tumor antigen, MUC1. Although tapasin has been previously shown by others to increase immune recognition of particular antigens, our results suggest that tapasin has a negative impact on the presentation of an immunodominant epitope from a specific model tumor antigen.

Original languageEnglish (US)
Pages (from-to)462-470
Number of pages9
JournalJournal of Clinical Immunology
Volume24
Issue number4
DOIs
StatePublished - Jul 1 2004

Fingerprint

Neoplasm Antigens
Major Histocompatibility Complex
Immunodominant Epitopes
Neoplasms
Peptides
Tumor Cell Line
Interferons
Transfection
Down-Regulation
tapasin
T-Lymphocytes
Antigens
Proteins

Keywords

  • Antigen presentation
  • MHC
  • Pancreatic cancer
  • Tapasin
  • Tumor antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Tapasin decreases immune responsiveness to a model tumor antigen. / Turnquist, Heth R.; Kohlgraf, Karl G.; McIlhaney, Mary M.; Mosley, R Lee; Hollingsworth, Michael A; Solheim, Joyce C.

In: Journal of Clinical Immunology, Vol. 24, No. 4, 01.07.2004, p. 462-470.

Research output: Contribution to journalArticle

Turnquist, Heth R. ; Kohlgraf, Karl G. ; McIlhaney, Mary M. ; Mosley, R Lee ; Hollingsworth, Michael A ; Solheim, Joyce C. / Tapasin decreases immune responsiveness to a model tumor antigen. In: Journal of Clinical Immunology. 2004 ; Vol. 24, No. 4. pp. 462-470.
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