T cell receptor signaling can directly enhance the avidity of CD28 ligand binding

Mariano Sanchez-Lockhart, Ana V. Rojas, Margaret M. Fettis, Richard Bauserman, Trissha R. Higa, Hongyu Miao, Richard E. Waugh, Jim Miller

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

T cell activation takes place in the context of a spatial and kinetic reorganization of cell surface proteins and signaling molecules at the contact site with an antigen presenting cell, termed the immunological synapse. Coordination of the activation, recruitment, and signaling from T cell receptor (TCR) in conjunction with adhesion and costimulatory receptors regulates both the initiation and duration of signaling that is required for T cell activation. The costimulatory receptor, CD28, is an essential signaling molecule that determines the quality and quantity of T cell immune responses. Although the functional consequences of CD28 engagement are well described, the molecular mechanisms that regulate CD28 function are largely unknown. Using a micropipet adhesion frequency assay, we show that TCR signaling enhances the direct binding between CD28 and its ligand, CD80. Although CD28 is expressed as a homodimer, soluble recombinant CD28 can only bind ligand monovalently. Our data suggest that the increase in CD28-CD28 binding is mediated through a change in CD28 valency. Molecular dynamic simulations and in vitro mutagenesis indicate that mutations at the base of the CD28 homodimer interface, distal to the ligand-binding site, can induce a change in the orientation of the dimer that allows for bivalent ligand binding. When expressed in T cells, this mutation allows for high avidity CD28-CD80 interactions without TCR signaling. Molecular dynamic simulations also suggest that wild type CD28 can stably adopt a bivalent conformation. These results support a model whereby inside-out signaling from the TCR can enhance CD28 ligand interactions by inducing a change in the CD28 dimer interface to allow for bivalent ligand binding and ultimately the transduction of CD28 costimulatory signals that are required for T cell activation.

Original languageEnglish (US)
Article numbere89263
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 24 2014

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T-Cell Antigen Receptor
T-cells
T-lymphocytes
Ligands
receptors
T-Lymphocytes
Chemical activation
Molecular Dynamics Simulation
Dimers
molecular dynamics
Molecular dynamics
Immunological Synapses
Adhesion
Mutation
adhesion
Antigen-Presenting Cells
Mutagenesis
Molecules
Computer simulation
mutation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sanchez-Lockhart, M., Rojas, A. V., Fettis, M. M., Bauserman, R., Higa, T. R., Miao, H., ... Miller, J. (2014). T cell receptor signaling can directly enhance the avidity of CD28 ligand binding. PloS one, 9(2), [e89263]. https://doi.org/10.1371/journal.pone.0089263

T cell receptor signaling can directly enhance the avidity of CD28 ligand binding. / Sanchez-Lockhart, Mariano; Rojas, Ana V.; Fettis, Margaret M.; Bauserman, Richard; Higa, Trissha R.; Miao, Hongyu; Waugh, Richard E.; Miller, Jim.

In: PloS one, Vol. 9, No. 2, e89263, 24.02.2014.

Research output: Contribution to journalArticle

Sanchez-Lockhart, M, Rojas, AV, Fettis, MM, Bauserman, R, Higa, TR, Miao, H, Waugh, RE & Miller, J 2014, 'T cell receptor signaling can directly enhance the avidity of CD28 ligand binding', PloS one, vol. 9, no. 2, e89263. https://doi.org/10.1371/journal.pone.0089263
Sanchez-Lockhart, Mariano ; Rojas, Ana V. ; Fettis, Margaret M. ; Bauserman, Richard ; Higa, Trissha R. ; Miao, Hongyu ; Waugh, Richard E. ; Miller, Jim. / T cell receptor signaling can directly enhance the avidity of CD28 ligand binding. In: PloS one. 2014 ; Vol. 9, No. 2.
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