T-cell Co-regulatory Molecule Expression in Renal Angiomyolipoma and Pulmonary Lymphangioleiomyomatosis

Stephen A. Boorjian, Yuri Sheinin, Paul L. Crispen, Christine M. Lohse, Bradley C. Leibovich, Eugene D. Kwon

Research output: Contribution to journalArticle

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Abstract

Objectives: To investigate the expression of B7-H3 and B7-H1 in renal angiomyolipoma (AML) tumors and the related, devastating syndrome of pulmonary lymphangioleiomyomatosis (LAM). We recently reported the high expression of T-cell co-regulatory B7-H ligands in renal cell carcinoma tumor vasculature and tumor cells. AML is a highly vascular tumor that most frequently emanates from the kidney. Events leading to its pathogenesis remain enigmatic and understudied. Methods: Immunohistochemical methods were used to assess the tumor expression of B7-H1 and B7-H3 in paraffin-embedded tissues from 110 patients who had undergone partial or radical nephrectomy for renal AML and from 7 patients with LAM who had undergone lung biopsy. Results: B7-H3 was expressed by 100% of the AML and LAM specimens, and B7-H1 expression was detected in only 2.7% of the specimens studied. Both membranous and cytoplasmic B7-H3 expression was noted in the smooth muscle, blood vessel, and lipoid cell components of the tumors; however, no expression was detected in the adjacent, normal parenchyma tissue. B7-H3 staining was noted in a median of 90% (range 20%-100%) of cells from renal AMLs and was independent of patient age (P = .43), sex (P = .27), tumor size (P = .21), and symptomatic presentation (P = .35). Conclusions: B7-H3 was expressed at high levels in renal AMLs and pulmonary LAM, and B7-H1 was infrequently expressed in these tumors. Additional studies are needed to evaluate the utility of B7-H3 as a diagnostic marker or immune/angiogenic target to improve the management of AML and the potentially devastating condition of LAM, for which effective treatment is lacking.

Original languageEnglish (US)
Pages (from-to)1359-1364
Number of pages6
JournalUrology
Volume74
Issue number6
DOIs
StatePublished - Dec 1 2009

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Lymphangioleiomyomatosis
Angiomyolipoma
Regulatory T-Lymphocytes
Kidney
Lung
Neoplasms
Blood Vessels
Cellular Structures
Nephrectomy
Renal Cell Carcinoma
Paraffin
Smooth Muscle
Biomarkers
Staining and Labeling
Ligands
Biopsy

ASJC Scopus subject areas

  • Urology

Cite this

Boorjian, S. A., Sheinin, Y., Crispen, P. L., Lohse, C. M., Leibovich, B. C., & Kwon, E. D. (2009). T-cell Co-regulatory Molecule Expression in Renal Angiomyolipoma and Pulmonary Lymphangioleiomyomatosis. Urology, 74(6), 1359-1364. https://doi.org/10.1016/j.urology.2009.03.007

T-cell Co-regulatory Molecule Expression in Renal Angiomyolipoma and Pulmonary Lymphangioleiomyomatosis. / Boorjian, Stephen A.; Sheinin, Yuri; Crispen, Paul L.; Lohse, Christine M.; Leibovich, Bradley C.; Kwon, Eugene D.

In: Urology, Vol. 74, No. 6, 01.12.2009, p. 1359-1364.

Research output: Contribution to journalArticle

Boorjian, SA, Sheinin, Y, Crispen, PL, Lohse, CM, Leibovich, BC & Kwon, ED 2009, 'T-cell Co-regulatory Molecule Expression in Renal Angiomyolipoma and Pulmonary Lymphangioleiomyomatosis', Urology, vol. 74, no. 6, pp. 1359-1364. https://doi.org/10.1016/j.urology.2009.03.007
Boorjian, Stephen A. ; Sheinin, Yuri ; Crispen, Paul L. ; Lohse, Christine M. ; Leibovich, Bradley C. ; Kwon, Eugene D. / T-cell Co-regulatory Molecule Expression in Renal Angiomyolipoma and Pulmonary Lymphangioleiomyomatosis. In: Urology. 2009 ; Vol. 74, No. 6. pp. 1359-1364.
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abstract = "Objectives: To investigate the expression of B7-H3 and B7-H1 in renal angiomyolipoma (AML) tumors and the related, devastating syndrome of pulmonary lymphangioleiomyomatosis (LAM). We recently reported the high expression of T-cell co-regulatory B7-H ligands in renal cell carcinoma tumor vasculature and tumor cells. AML is a highly vascular tumor that most frequently emanates from the kidney. Events leading to its pathogenesis remain enigmatic and understudied. Methods: Immunohistochemical methods were used to assess the tumor expression of B7-H1 and B7-H3 in paraffin-embedded tissues from 110 patients who had undergone partial or radical nephrectomy for renal AML and from 7 patients with LAM who had undergone lung biopsy. Results: B7-H3 was expressed by 100{\%} of the AML and LAM specimens, and B7-H1 expression was detected in only 2.7{\%} of the specimens studied. Both membranous and cytoplasmic B7-H3 expression was noted in the smooth muscle, blood vessel, and lipoid cell components of the tumors; however, no expression was detected in the adjacent, normal parenchyma tissue. B7-H3 staining was noted in a median of 90{\%} (range 20{\%}-100{\%}) of cells from renal AMLs and was independent of patient age (P = .43), sex (P = .27), tumor size (P = .21), and symptomatic presentation (P = .35). Conclusions: B7-H3 was expressed at high levels in renal AMLs and pulmonary LAM, and B7-H1 was infrequently expressed in these tumors. Additional studies are needed to evaluate the utility of B7-H3 as a diagnostic marker or immune/angiogenic target to improve the management of AML and the potentially devastating condition of LAM, for which effective treatment is lacking.",
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AU - Boorjian, Stephen A.

AU - Sheinin, Yuri

AU - Crispen, Paul L.

AU - Lohse, Christine M.

AU - Leibovich, Bradley C.

AU - Kwon, Eugene D.

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N2 - Objectives: To investigate the expression of B7-H3 and B7-H1 in renal angiomyolipoma (AML) tumors and the related, devastating syndrome of pulmonary lymphangioleiomyomatosis (LAM). We recently reported the high expression of T-cell co-regulatory B7-H ligands in renal cell carcinoma tumor vasculature and tumor cells. AML is a highly vascular tumor that most frequently emanates from the kidney. Events leading to its pathogenesis remain enigmatic and understudied. Methods: Immunohistochemical methods were used to assess the tumor expression of B7-H1 and B7-H3 in paraffin-embedded tissues from 110 patients who had undergone partial or radical nephrectomy for renal AML and from 7 patients with LAM who had undergone lung biopsy. Results: B7-H3 was expressed by 100% of the AML and LAM specimens, and B7-H1 expression was detected in only 2.7% of the specimens studied. Both membranous and cytoplasmic B7-H3 expression was noted in the smooth muscle, blood vessel, and lipoid cell components of the tumors; however, no expression was detected in the adjacent, normal parenchyma tissue. B7-H3 staining was noted in a median of 90% (range 20%-100%) of cells from renal AMLs and was independent of patient age (P = .43), sex (P = .27), tumor size (P = .21), and symptomatic presentation (P = .35). Conclusions: B7-H3 was expressed at high levels in renal AMLs and pulmonary LAM, and B7-H1 was infrequently expressed in these tumors. Additional studies are needed to evaluate the utility of B7-H3 as a diagnostic marker or immune/angiogenic target to improve the management of AML and the potentially devastating condition of LAM, for which effective treatment is lacking.

AB - Objectives: To investigate the expression of B7-H3 and B7-H1 in renal angiomyolipoma (AML) tumors and the related, devastating syndrome of pulmonary lymphangioleiomyomatosis (LAM). We recently reported the high expression of T-cell co-regulatory B7-H ligands in renal cell carcinoma tumor vasculature and tumor cells. AML is a highly vascular tumor that most frequently emanates from the kidney. Events leading to its pathogenesis remain enigmatic and understudied. Methods: Immunohistochemical methods were used to assess the tumor expression of B7-H1 and B7-H3 in paraffin-embedded tissues from 110 patients who had undergone partial or radical nephrectomy for renal AML and from 7 patients with LAM who had undergone lung biopsy. Results: B7-H3 was expressed by 100% of the AML and LAM specimens, and B7-H1 expression was detected in only 2.7% of the specimens studied. Both membranous and cytoplasmic B7-H3 expression was noted in the smooth muscle, blood vessel, and lipoid cell components of the tumors; however, no expression was detected in the adjacent, normal parenchyma tissue. B7-H3 staining was noted in a median of 90% (range 20%-100%) of cells from renal AMLs and was independent of patient age (P = .43), sex (P = .27), tumor size (P = .21), and symptomatic presentation (P = .35). Conclusions: B7-H3 was expressed at high levels in renal AMLs and pulmonary LAM, and B7-H1 was infrequently expressed in these tumors. Additional studies are needed to evaluate the utility of B7-H3 as a diagnostic marker or immune/angiogenic target to improve the management of AML and the potentially devastating condition of LAM, for which effective treatment is lacking.

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