Systemically delivered insulin-like growth factor-I enhances mesenchymal stem cell-dependent fracture healing

Timothy J. Myers, Yun Yan, Froilan Granero-Molto, Jared A. Weis, Lara Longobardi, Tieshi Li, Ying Li, Clara Contaldo, Huseyin Ozkhan, Anna Spagnoli

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study, we examined the effectiveness of systemic subcutaneous delivery of recombinant Insulin-like growth factor (IGF)-I concurrently with primary cultured bone marrow-derived mesenchymal stem cell (MSC) transplant on fracture repair. We found that the fracture callus volume increased in mice with a stabilized tibia fracture that received IGF-IMSC when compared with that in either untreated or MSC alone treated mice. In evaluating the callus tissue components, we found that the soft and new bone tissue volumes were significantly increased in IGF-IMSC recipients. Histological and in-situ hybridization analyses confirmed a characteristic increase of newly forming bone in IGF-IMSC recipients and that healing progressed mostly through endochondral ossification. The increase in soft and new bone tissue volumes correlated with increased force and toughness as determined by biomechanical testing. In conclusion, MSC transplant concurrent with systemic delivery of IGF-I improves fracture repair suggesting that IGF-IMSC could be a novel therapeutic approach in patients who have inadequate fracture repair.

Original languageEnglish (US)
Pages (from-to)230-241
Number of pages12
JournalGrowth Factors
Volume30
Issue number4
DOIs
StatePublished - Aug 2012

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Fracture Healing
Somatomedins
Stem cells
Mesenchymal Stromal Cells
Insulin-Like Growth Factor I
Bone
Bony Callus
Transplants
Bone and Bones
Repair
Tissue
Tibia
Osteogenesis
In Situ Hybridization
Bone Marrow
Toughness
Testing
Therapeutics

Keywords

  • Endochondral ossification
  • Fracture healing
  • Insulin-like growth factor-I
  • Mesenchymal stem cells
  • Non-unions
  • Regenerative medicine

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Systemically delivered insulin-like growth factor-I enhances mesenchymal stem cell-dependent fracture healing. / Myers, Timothy J.; Yan, Yun; Granero-Molto, Froilan; Weis, Jared A.; Longobardi, Lara; Li, Tieshi; Li, Ying; Contaldo, Clara; Ozkhan, Huseyin; Spagnoli, Anna.

In: Growth Factors, Vol. 30, No. 4, 08.2012, p. 230-241.

Research output: Contribution to journalArticle

Myers, TJ, Yan, Y, Granero-Molto, F, Weis, JA, Longobardi, L, Li, T, Li, Y, Contaldo, C, Ozkhan, H & Spagnoli, A 2012, 'Systemically delivered insulin-like growth factor-I enhances mesenchymal stem cell-dependent fracture healing', Growth Factors, vol. 30, no. 4, pp. 230-241. https://doi.org/10.3109/08977194.2012.683188
Myers, Timothy J. ; Yan, Yun ; Granero-Molto, Froilan ; Weis, Jared A. ; Longobardi, Lara ; Li, Tieshi ; Li, Ying ; Contaldo, Clara ; Ozkhan, Huseyin ; Spagnoli, Anna. / Systemically delivered insulin-like growth factor-I enhances mesenchymal stem cell-dependent fracture healing. In: Growth Factors. 2012 ; Vol. 30, No. 4. pp. 230-241.
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