Abstract
Recently, confirmed occurrences of persistent bovine viral diarrhea virus (BVDV) infection in North American alpacas have raised concerns about the role of persistently infected (PI) alpacas in transmission of virus among herds, yet only limited pathological descriptions of persistent infections in alpacas have been reported. The objective of this study was to characterize BVDV antigen distribution in 10 PI alpacas of varying age and to compare viral antigen distribution and localization in tissues of PI alpacas with 5 PI calves of varying age. Ocular dysplasia was evident in 1 PI alpaca, constituting the first reported congenital ocular lesion in PI alpacas. Viral antigen was widely distributed in alpaca tissues and was prominent in neurons, endothelial cells, and vascular tunica media myocytes but had limited distribution in lymphoid tissues and moderate distribution in epithelium of several organ systems of alpacas. Macrophages in the alpaca gastrointestinal system submucosa and lymph node medullary sinuses often had prominent labeling. In addition, only 1 alpaca had antigen labeling in the bone marrow in contrast to PI cattle. Labeled cells in calf tissues were more widely distributed, occurring prominently in lymphoid and epithelial tissues. Common features of the 2 host species were widespread antigen labeling and absence of lymphoid depletion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 308-317 |
| Number of pages | 10 |
| Journal | Veterinary pathology |
| Volume | 50 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 13 2013 |
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Keywords
- New World
- alpacas
- bovine pestivirus
- bovine viral diarrhea virus
- camelids
- immunohistochemistry
- persistent infection
- systemic
ASJC Scopus subject areas
- veterinary(all)
Cite this
Systemic Distribution of Viral Antigen in Alpacas Persistently Infected With Bovine Pestivirus. / Henningson, J. N.; Steffen, David J; Topliff, C. L.; Kurth, K. L.; Dubielzig, R. R.; Brodersen, B. W.; Bedenice, D.; Eskridge, Kent M; Callan, R. J.; Reggiardo, C.; Rupp, G. P.; Kelling, Clayton L.
In: Veterinary pathology, Vol. 50, No. 2, 13.03.2013, p. 308-317.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Systemic Distribution of Viral Antigen in Alpacas Persistently Infected With Bovine Pestivirus
AU - Henningson, J. N.
AU - Steffen, David J
AU - Topliff, C. L.
AU - Kurth, K. L.
AU - Dubielzig, R. R.
AU - Brodersen, B. W.
AU - Bedenice, D.
AU - Eskridge, Kent M
AU - Callan, R. J.
AU - Reggiardo, C.
AU - Rupp, G. P.
AU - Kelling, Clayton L
PY - 2013/3/13
Y1 - 2013/3/13
N2 - Recently, confirmed occurrences of persistent bovine viral diarrhea virus (BVDV) infection in North American alpacas have raised concerns about the role of persistently infected (PI) alpacas in transmission of virus among herds, yet only limited pathological descriptions of persistent infections in alpacas have been reported. The objective of this study was to characterize BVDV antigen distribution in 10 PI alpacas of varying age and to compare viral antigen distribution and localization in tissues of PI alpacas with 5 PI calves of varying age. Ocular dysplasia was evident in 1 PI alpaca, constituting the first reported congenital ocular lesion in PI alpacas. Viral antigen was widely distributed in alpaca tissues and was prominent in neurons, endothelial cells, and vascular tunica media myocytes but had limited distribution in lymphoid tissues and moderate distribution in epithelium of several organ systems of alpacas. Macrophages in the alpaca gastrointestinal system submucosa and lymph node medullary sinuses often had prominent labeling. In addition, only 1 alpaca had antigen labeling in the bone marrow in contrast to PI cattle. Labeled cells in calf tissues were more widely distributed, occurring prominently in lymphoid and epithelial tissues. Common features of the 2 host species were widespread antigen labeling and absence of lymphoid depletion.
AB - Recently, confirmed occurrences of persistent bovine viral diarrhea virus (BVDV) infection in North American alpacas have raised concerns about the role of persistently infected (PI) alpacas in transmission of virus among herds, yet only limited pathological descriptions of persistent infections in alpacas have been reported. The objective of this study was to characterize BVDV antigen distribution in 10 PI alpacas of varying age and to compare viral antigen distribution and localization in tissues of PI alpacas with 5 PI calves of varying age. Ocular dysplasia was evident in 1 PI alpaca, constituting the first reported congenital ocular lesion in PI alpacas. Viral antigen was widely distributed in alpaca tissues and was prominent in neurons, endothelial cells, and vascular tunica media myocytes but had limited distribution in lymphoid tissues and moderate distribution in epithelium of several organ systems of alpacas. Macrophages in the alpaca gastrointestinal system submucosa and lymph node medullary sinuses often had prominent labeling. In addition, only 1 alpaca had antigen labeling in the bone marrow in contrast to PI cattle. Labeled cells in calf tissues were more widely distributed, occurring prominently in lymphoid and epithelial tissues. Common features of the 2 host species were widespread antigen labeling and absence of lymphoid depletion.
KW - New World
KW - alpacas
KW - bovine pestivirus
KW - bovine viral diarrhea virus
KW - camelids
KW - immunohistochemistry
KW - persistent infection
KW - systemic
UR - http://www.scopus.com/inward/record.url?scp=84874770298&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874770298&partnerID=8YFLogxK
U2 - 10.1177/0300985812447827
DO - 10.1177/0300985812447827
M3 - Article
C2 - 22688584
AN - SCOPUS:84874770298
VL - 50
SP - 308
EP - 317
JO - Veterinary Pathology
JF - Veterinary Pathology
SN - 0300-9858
IS - 2
ER -