Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela, Catalina Amador, Manuel A. Patarroyo

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume59
Issue number1
DOIs
Publication statusPublished - Sep 1 2007

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Keywords

  • Cervical cancer
  • Genotyping
  • Human papillomavirus
  • Synthetic genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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