Synthetic genes

a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela, Catalina Amador, Manuel A. Patarroyo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume59
Issue number1
DOIs
StatePublished - Sep 1 2007

Fingerprint

Synthetic Genes
Genotype
Polymerase Chain Reaction
Human papillomavirus 31
Human papillomavirus 18
Papillomavirus Infections
Viral Genes
Routine Diagnostic Tests
Oligonucleotides

Keywords

  • Cervical cancer
  • Genotyping
  • Human papillomavirus
  • Synthetic genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Synthetic genes : a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays. / Orjuela, Karen; Amador, Catalina; Patarroyo, Manuel A.

In: Diagnostic Microbiology and Infectious Disease, Vol. 59, No. 1, 01.09.2007, p. 101-104.

Research output: Contribution to journalArticle

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