Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro

S. M. Kharbanda, V. Band, K. Murugesan, U. Verma, V. L. Bhargava, A. Farooq

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effect of synthetic antiprogestins, RMI 14156 and STS 557, on gonadotrophin stimulated estradiol (E2) production in human ovarian granulosa cells (GC) has been tested in vitro, to see if by virtue of their antiprogestational activity these compounds would promote the production of E2, and thereby follicular growth. Granulosa cells from human ovaries were cultured in modified minimum essential medium with or without luteinising hormone (LH, 10 ng/ml), follicle stimulating hormone (FSH, 100 ng/ml), androstenedione (A, 10-7 M), RMI 14156 (10-9 M - 10-5 M) and STS 557 (10-9 M - 10-5 M), in a humidified 95% air 5% CO2 atmosphere at 37°C for 48 hours. RMI 14156 and STS 557 were found to stimulate the production of E2 by cultured human GC in a dose dependent manner, the stimulation being significant (P < 0.001 - P < 0.05) at higher doses (10-6 M, 10-5 M). The two antiprogestins used also stimulated the gonadotrophin induced E2 production significantly. Results demonstrated that these synthetic antiprogestins, having an 'estra' type of basic structure RMI 14156 (17β hydroxy-7α-methyl-5-estra-en-3-one); STS 557 (17α-cyanomethyl-17β-hydroxy-estra-4,9(10)diene-3-one) can directly promote E2 production from human ovarian GC in vitro.

Original languageEnglish (US)
Pages (from-to)174-180
Number of pages7
JournalExperimental and Clinical Endocrinology
Volume89
Issue number2
StatePublished - Jul 13 1987

Fingerprint

dienogest
Granulosa Cells
Estradiol
Gonadotropins
Androstenedione
Follicle Stimulating Hormone
Luteinizing Hormone
Atmosphere
Ovary
Air
In Vitro Techniques
17 beta-hydroxy-7 alpha-methyl-5-estren-3-one
Growth

ASJC Scopus subject areas

  • Endocrinology

Cite this

Kharbanda, S. M., Band, V., Murugesan, K., Verma, U., Bhargava, V. L., & Farooq, A. (1987). Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro. Experimental and Clinical Endocrinology, 89(2), 174-180.

Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro. / Kharbanda, S. M.; Band, V.; Murugesan, K.; Verma, U.; Bhargava, V. L.; Farooq, A.

In: Experimental and Clinical Endocrinology, Vol. 89, No. 2, 13.07.1987, p. 174-180.

Research output: Contribution to journalArticle

Kharbanda, SM, Band, V, Murugesan, K, Verma, U, Bhargava, VL & Farooq, A 1987, 'Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro', Experimental and Clinical Endocrinology, vol. 89, no. 2, pp. 174-180.
Kharbanda SM, Band V, Murugesan K, Verma U, Bhargava VL, Farooq A. Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro. Experimental and Clinical Endocrinology. 1987 Jul 13;89(2):174-180.
Kharbanda, S. M. ; Band, V. ; Murugesan, K. ; Verma, U. ; Bhargava, V. L. ; Farooq, A. / Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro. In: Experimental and Clinical Endocrinology. 1987 ; Vol. 89, No. 2. pp. 174-180.
@article{e499bdfeea3b4b71ad7ff3968964eb40,
title = "Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro",
abstract = "The effect of synthetic antiprogestins, RMI 14156 and STS 557, on gonadotrophin stimulated estradiol (E2) production in human ovarian granulosa cells (GC) has been tested in vitro, to see if by virtue of their antiprogestational activity these compounds would promote the production of E2, and thereby follicular growth. Granulosa cells from human ovaries were cultured in modified minimum essential medium with or without luteinising hormone (LH, 10 ng/ml), follicle stimulating hormone (FSH, 100 ng/ml), androstenedione (A, 10-7 M), RMI 14156 (10-9 M - 10-5 M) and STS 557 (10-9 M - 10-5 M), in a humidified 95{\%} air 5{\%} CO2 atmosphere at 37°C for 48 hours. RMI 14156 and STS 557 were found to stimulate the production of E2 by cultured human GC in a dose dependent manner, the stimulation being significant (P < 0.001 - P < 0.05) at higher doses (10-6 M, 10-5 M). The two antiprogestins used also stimulated the gonadotrophin induced E2 production significantly. Results demonstrated that these synthetic antiprogestins, having an 'estra' type of basic structure RMI 14156 (17β hydroxy-7α-methyl-5-estra-en-3-one); STS 557 (17α-cyanomethyl-17β-hydroxy-estra-4,9(10)diene-3-one) can directly promote E2 production from human ovarian GC in vitro.",
author = "Kharbanda, {S. M.} and V. Band and K. Murugesan and U. Verma and Bhargava, {V. L.} and A. Farooq",
year = "1987",
month = "7",
day = "13",
language = "English (US)",
volume = "89",
pages = "174--180",
journal = "Experimental and Clinical Endocrinology and Diabetes",
issn = "0947-7349",
publisher = "Thieme",
number = "2",

}

TY - JOUR

T1 - Synthetic antiprogestins stimulate human granulosa cell estradiol production in vitro

AU - Kharbanda, S. M.

AU - Band, V.

AU - Murugesan, K.

AU - Verma, U.

AU - Bhargava, V. L.

AU - Farooq, A.

PY - 1987/7/13

Y1 - 1987/7/13

N2 - The effect of synthetic antiprogestins, RMI 14156 and STS 557, on gonadotrophin stimulated estradiol (E2) production in human ovarian granulosa cells (GC) has been tested in vitro, to see if by virtue of their antiprogestational activity these compounds would promote the production of E2, and thereby follicular growth. Granulosa cells from human ovaries were cultured in modified minimum essential medium with or without luteinising hormone (LH, 10 ng/ml), follicle stimulating hormone (FSH, 100 ng/ml), androstenedione (A, 10-7 M), RMI 14156 (10-9 M - 10-5 M) and STS 557 (10-9 M - 10-5 M), in a humidified 95% air 5% CO2 atmosphere at 37°C for 48 hours. RMI 14156 and STS 557 were found to stimulate the production of E2 by cultured human GC in a dose dependent manner, the stimulation being significant (P < 0.001 - P < 0.05) at higher doses (10-6 M, 10-5 M). The two antiprogestins used also stimulated the gonadotrophin induced E2 production significantly. Results demonstrated that these synthetic antiprogestins, having an 'estra' type of basic structure RMI 14156 (17β hydroxy-7α-methyl-5-estra-en-3-one); STS 557 (17α-cyanomethyl-17β-hydroxy-estra-4,9(10)diene-3-one) can directly promote E2 production from human ovarian GC in vitro.

AB - The effect of synthetic antiprogestins, RMI 14156 and STS 557, on gonadotrophin stimulated estradiol (E2) production in human ovarian granulosa cells (GC) has been tested in vitro, to see if by virtue of their antiprogestational activity these compounds would promote the production of E2, and thereby follicular growth. Granulosa cells from human ovaries were cultured in modified minimum essential medium with or without luteinising hormone (LH, 10 ng/ml), follicle stimulating hormone (FSH, 100 ng/ml), androstenedione (A, 10-7 M), RMI 14156 (10-9 M - 10-5 M) and STS 557 (10-9 M - 10-5 M), in a humidified 95% air 5% CO2 atmosphere at 37°C for 48 hours. RMI 14156 and STS 557 were found to stimulate the production of E2 by cultured human GC in a dose dependent manner, the stimulation being significant (P < 0.001 - P < 0.05) at higher doses (10-6 M, 10-5 M). The two antiprogestins used also stimulated the gonadotrophin induced E2 production significantly. Results demonstrated that these synthetic antiprogestins, having an 'estra' type of basic structure RMI 14156 (17β hydroxy-7α-methyl-5-estra-en-3-one); STS 557 (17α-cyanomethyl-17β-hydroxy-estra-4,9(10)diene-3-one) can directly promote E2 production from human ovarian GC in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0023264516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023264516&partnerID=8YFLogxK

M3 - Article

C2 - 3111872

AN - SCOPUS:0023264516

VL - 89

SP - 174

EP - 180

JO - Experimental and Clinical Endocrinology and Diabetes

JF - Experimental and Clinical Endocrinology and Diabetes

SN - 0947-7349

IS - 2

ER -