Synthesis of starlike N-(2-hydroxypropyl)methacrylamide copolymers: Potential drug carriers

Dong Wang, Pavla Kopečková, Tamara Minko, Vajira Nanayakkara, Jindřich Kopeček

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Starlike HPMA copolymers were synthesized by conjugating semitelechelic poly[N-(2-hydroxypropyl)-methacrylamide] macromolecules (ST-PHPMA, arm) with PAMAM dendrimers (core: G2, G3, G4). ST-PHPMA was synthesized by chain transfer free radical polymerization, and the terminal -COOH was activated with N-hydroxysuccinimide. Doxorubicin (DOX) was introduced into the starlike HPMA copolymer to evaluate its potential as a drug delivery system. The polymers were characterized with SEC, NMR, and UV. Cytotoxicity of the DOX containing starlike HPMA copolymer was determined on an A2780 human ovarian carcinoma cell line and compared with DOX-containing linear HPMA copolymers. The rate of in vitro DOX release from polymer-DOX conjugates in the presence of cathepsin B (CP-B, lysosomal cysteine proteinase) was determined and correlated with cytotoxicity results.

Original languageEnglish (US)
Pages (from-to)313-319
Number of pages7
JournalBiomacromolecules
Volume1
Issue number3
StatePublished - Dec 1 2000

Fingerprint

hydroxypropyl methacrylate
Drug Carriers
Doxorubicin
Copolymers
Cytotoxicity
Polymers
Dendrimers
Free radical polymerization
Cathepsin B
Macromolecules
Cysteine Proteases
Drug Delivery Systems
Polymerization
Cells
Nuclear magnetic resonance
Free Radicals
N-(2-hydroxypropyl)methacrylamide
Carcinoma
Cell Line

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Wang, D., Kopečková, P., Minko, T., Nanayakkara, V., & Kopeček, J. (2000). Synthesis of starlike N-(2-hydroxypropyl)methacrylamide copolymers: Potential drug carriers. Biomacromolecules, 1(3), 313-319.

Synthesis of starlike N-(2-hydroxypropyl)methacrylamide copolymers : Potential drug carriers. / Wang, Dong; Kopečková, Pavla; Minko, Tamara; Nanayakkara, Vajira; Kopeček, Jindřich.

In: Biomacromolecules, Vol. 1, No. 3, 01.12.2000, p. 313-319.

Research output: Contribution to journalArticle

Wang, D, Kopečková, P, Minko, T, Nanayakkara, V & Kopeček, J 2000, 'Synthesis of starlike N-(2-hydroxypropyl)methacrylamide copolymers: Potential drug carriers', Biomacromolecules, vol. 1, no. 3, pp. 313-319.
Wang D, Kopečková P, Minko T, Nanayakkara V, Kopeček J. Synthesis of starlike N-(2-hydroxypropyl)methacrylamide copolymers: Potential drug carriers. Biomacromolecules. 2000 Dec 1;1(3):313-319.
Wang, Dong ; Kopečková, Pavla ; Minko, Tamara ; Nanayakkara, Vajira ; Kopeček, Jindřich. / Synthesis of starlike N-(2-hydroxypropyl)methacrylamide copolymers : Potential drug carriers. In: Biomacromolecules. 2000 ; Vol. 1, No. 3. pp. 313-319.
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