Synthesis of polyadenylate-containing RNA during the germination of Rhizopus stolonifer sporangiospores

Shelby N. Freer, Mari Mayama, James L. Van Etten

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Rhizopus stolonifer sporangiospores synthesized polyadenylate-containing [poly(A+)]RNA de novo during the first 15 min of germination. The length of the poly(A) segments isolated from total RNA gradually decreased from about 100 adenylate residues for spores pulse-labeled during the first 15 min of germination to 60 adenylate residues for spores pulse-labeled for 15 min at 4 h into germination. In contrast, the average length of the newly synthesized poly(A) segments isolated from polysomal RNA[poly(A+)mRNA] was about 50 adenylic acid residues at all pulse periods. The average size of the poly(A+)RNA synthesized during the first 15 min of germination was larger (ca. 1200 nucleotides) than the poly(A+)mRNA (ca. 860 nucleotides). The results are consistent with the hypothesis that the poly(A+)RNA synthesized early in germination is predominantly a precursor of poly(A+)mRNA. As germination proceeds, precursor poly(A+)RNA is processed more rapidly into poly(A+)mRNA and, thus, the majority of the poly(A+)RNA isolated from total RNA at later stages of germination consists of poly(A+)mRNA. If our hypothesis is correct, the processing of precursor poly(A+)mRNA into poly(A+)mRNA involves cleavage of the precursor molecule at both the 5′ and 3′ ends; about 50 adenylic acid residues are removed from the 3′ end and 300 nucleotides are removed from the 5′ end.

Original languageEnglish (US)
Pages (from-to)116-127
Number of pages12
JournalExperimental Mycology
Volume1
Issue number2
DOIs
StatePublished - Jun 1977

Fingerprint

Rhizopus
Germination
RNA
Messenger RNA
Poly A
Nucleotides
Adenosine Monophosphate
Spores

Keywords

  • RNA, messenger, heterogeneous nuclear
  • Rhizopus stolonifer
  • poly(A)
  • sporangiospores
  • thallophytes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Synthesis of polyadenylate-containing RNA during the germination of Rhizopus stolonifer sporangiospores. / Freer, Shelby N.; Mayama, Mari; Van Etten, James L.

In: Experimental Mycology, Vol. 1, No. 2, 06.1977, p. 116-127.

Research output: Contribution to journalArticle

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AB - Rhizopus stolonifer sporangiospores synthesized polyadenylate-containing [poly(A+)]RNA de novo during the first 15 min of germination. The length of the poly(A) segments isolated from total RNA gradually decreased from about 100 adenylate residues for spores pulse-labeled during the first 15 min of germination to 60 adenylate residues for spores pulse-labeled for 15 min at 4 h into germination. In contrast, the average length of the newly synthesized poly(A) segments isolated from polysomal RNA[poly(A+)mRNA] was about 50 adenylic acid residues at all pulse periods. The average size of the poly(A+)RNA synthesized during the first 15 min of germination was larger (ca. 1200 nucleotides) than the poly(A+)mRNA (ca. 860 nucleotides). The results are consistent with the hypothesis that the poly(A+)RNA synthesized early in germination is predominantly a precursor of poly(A+)mRNA. As germination proceeds, precursor poly(A+)RNA is processed more rapidly into poly(A+)mRNA and, thus, the majority of the poly(A+)RNA isolated from total RNA at later stages of germination consists of poly(A+)mRNA. If our hypothesis is correct, the processing of precursor poly(A+)mRNA into poly(A+)mRNA involves cleavage of the precursor molecule at both the 5′ and 3′ ends; about 50 adenylic acid residues are removed from the 3′ end and 300 nucleotides are removed from the 5′ end.

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