Synthesis of fluorescein labeled 7-methylguanosinemonophosphate

Amarnath Natarajan, Nathan Moerke, Yun Hua Fan, Han Chen, William J. Christ, Gerhard Wagner, Jose A. Halperin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Binding of eIF4E to the cap structure (m7GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence- polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.

Original languageEnglish (US)
Pages (from-to)2657-2660
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number10
DOIs
StatePublished - May 17 2004

Fingerprint

Fluorescence Polarization
Fluorescein
Assays
Fluorescence
Polarization
Protein Biosynthesis
Messenger RNA
Molecules
Communication

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Natarajan, A., Moerke, N., Fan, Y. H., Chen, H., Christ, W. J., Wagner, G., & Halperin, J. A. (2004). Synthesis of fluorescein labeled 7-methylguanosinemonophosphate. Bioorganic and Medicinal Chemistry Letters, 14(10), 2657-2660. https://doi.org/10.1016/j.bmcl.2004.02.090

Synthesis of fluorescein labeled 7-methylguanosinemonophosphate. / Natarajan, Amarnath; Moerke, Nathan; Fan, Yun Hua; Chen, Han; Christ, William J.; Wagner, Gerhard; Halperin, Jose A.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 14, No. 10, 17.05.2004, p. 2657-2660.

Research output: Contribution to journalArticle

Natarajan, A, Moerke, N, Fan, YH, Chen, H, Christ, WJ, Wagner, G & Halperin, JA 2004, 'Synthesis of fluorescein labeled 7-methylguanosinemonophosphate', Bioorganic and Medicinal Chemistry Letters, vol. 14, no. 10, pp. 2657-2660. https://doi.org/10.1016/j.bmcl.2004.02.090
Natarajan, Amarnath ; Moerke, Nathan ; Fan, Yun Hua ; Chen, Han ; Christ, William J. ; Wagner, Gerhard ; Halperin, Jose A. / Synthesis of fluorescein labeled 7-methylguanosinemonophosphate. In: Bioorganic and Medicinal Chemistry Letters. 2004 ; Vol. 14, No. 10. pp. 2657-2660.
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