Synthesis of fluorescein labeled 7-methylguanosinemonophosphate

Amarnath Natarajan, Nathan Moerke, Yun Hua Fan, Han Chen, William J. Christ, Gerhard Wagner, Jose A. Halperin

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Binding of eIF4E to the cap structure (m7GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence- polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.

Original languageEnglish (US)
Pages (from-to)2657-2660
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number10
DOIs
StatePublished - May 17 2004

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Natarajan, A., Moerke, N., Fan, Y. H., Chen, H., Christ, W. J., Wagner, G., & Halperin, J. A. (2004). Synthesis of fluorescein labeled 7-methylguanosinemonophosphate. Bioorganic and Medicinal Chemistry Letters, 14(10), 2657-2660. https://doi.org/10.1016/j.bmcl.2004.02.090