Synthesis of fibronectin, laminin, and several collagens by a liver-derived epithelial line

J. M. Foidart, J. J. Berman, L. Paglia, S. Rennard, S. Abe, A. Perantoni, G. R. Martin

Research output: Contribution to journalArticle

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Abstract

We have investigated the ability of ARL-6 cells, a cell line derived from rat liver, to synthesize various collagens and two glycoproteins of the extracellular matrix, fibronectin, and laminin. Using immunofluorescence, we detected types I, III, and IV collagen plus laminin and fibronectin. Antibodies to types I and III collagen and to fibronectin were associated with most cells and showed a similar distribution. Type IV collagen and laminin were found in thin filaments associated with a small proportion of the cells. Chemical studies showed that ARL-6 cells synthesize predominantly types I and III collagens. The level of collagen synthesis was greatly affected by the presence of exogenous fibronectin added to the cells in the media. Cells maintained in fibronectin-free serum synthesized much less collagen. These studies indicate that liver-derived cells can synthesize a variety of connective tissue proteins and that collagen synthesis by these cells is enhanced by the presence of fibronectin.

Original languageEnglish (US)
Pages (from-to)525-532
Number of pages8
JournalLaboratory Investigation
Volume42
Issue number5
StatePublished - Jul 18 1980

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Laminin
Fibronectins
Collagen
Liver
Collagen Type III
Collagen Type I
Collagen Type IV
Connective Tissue
Fluorescent Antibody Technique
Extracellular Matrix
Glycoproteins
Cell Line
Antibodies
Serum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Foidart, J. M., Berman, J. J., Paglia, L., Rennard, S., Abe, S., Perantoni, A., & Martin, G. R. (1980). Synthesis of fibronectin, laminin, and several collagens by a liver-derived epithelial line. Laboratory Investigation, 42(5), 525-532.

Synthesis of fibronectin, laminin, and several collagens by a liver-derived epithelial line. / Foidart, J. M.; Berman, J. J.; Paglia, L.; Rennard, S.; Abe, S.; Perantoni, A.; Martin, G. R.

In: Laboratory Investigation, Vol. 42, No. 5, 18.07.1980, p. 525-532.

Research output: Contribution to journalArticle

Foidart, JM, Berman, JJ, Paglia, L, Rennard, S, Abe, S, Perantoni, A & Martin, GR 1980, 'Synthesis of fibronectin, laminin, and several collagens by a liver-derived epithelial line', Laboratory Investigation, vol. 42, no. 5, pp. 525-532.
Foidart JM, Berman JJ, Paglia L, Rennard S, Abe S, Perantoni A et al. Synthesis of fibronectin, laminin, and several collagens by a liver-derived epithelial line. Laboratory Investigation. 1980 Jul 18;42(5):525-532.
Foidart, J. M. ; Berman, J. J. ; Paglia, L. ; Rennard, S. ; Abe, S. ; Perantoni, A. ; Martin, G. R. / Synthesis of fibronectin, laminin, and several collagens by a liver-derived epithelial line. In: Laboratory Investigation. 1980 ; Vol. 42, No. 5. pp. 525-532.
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