Synthesis of DNA and protein by Anaplasma marginale in bovine erythrocytes during short term culture

W. C. Davis, James E Talmadge, S. M. Parish, M. I. Johnson, S. D. Vibber

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Bovine erythrocytes infected with A. marginale were cultured for 1 to 5 days in a CO 2 incubation chamber, pulse-labeled with [ 3H]thymidine and [ 14C]methionine, lysed, and fractionated by differential centrifugation and continuous density gradient centrifugation in Renografin. Anaplasma and associated fragments of stroma formed two distinct bands in the dense region of the gradient. Electron microscopic examination of pelleted material from the bands from cells cultured for 1 day revealed the presence of organisms that were morphologically intact or in various states of degeneration. Examination of fractions from the gradient for incorporation of label revealed that anaplasma present in erythrocytes can incorporate both [ 3H]thymidine and [ 14C]methionine. Subsequent experiments demonstrated that organisms cultured for 3 and 5 days incorporated the radiolabeled compounds also, but to a lesser extent. The experiments demonstrate that it is possible to culture anaplasma in vitro for short periods of time and monitor their growth characteristics.

Original languageEnglish (US)
Pages (from-to)597-602
Number of pages6
JournalInfection and immunity
Volume22
Issue number2
StatePublished - Dec 1 1978
Externally publishedYes

Fingerprint

Anaplasma
Anaplasma marginale
Erythrocytes
Methionine
Thymidine
DNA
Diatrizoate Meglumine
Proteins
Density Gradient Centrifugation
Carbon Monoxide
Centrifugation
Cultured Cells
Electrons
Growth

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Synthesis of DNA and protein by Anaplasma marginale in bovine erythrocytes during short term culture. / Davis, W. C.; Talmadge, James E; Parish, S. M.; Johnson, M. I.; Vibber, S. D.

In: Infection and immunity, Vol. 22, No. 2, 01.12.1978, p. 597-602.

Research output: Contribution to journalArticle

Davis, W. C. ; Talmadge, James E ; Parish, S. M. ; Johnson, M. I. ; Vibber, S. D. / Synthesis of DNA and protein by Anaplasma marginale in bovine erythrocytes during short term culture. In: Infection and immunity. 1978 ; Vol. 22, No. 2. pp. 597-602.
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