Synthesis of 2,5‐dihydroxy[carboxy‐14C]benzoic acid for electrochemical protein modification

Varghese John, Jonathan L. Vennerstrom, Thomas J. Holmes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The synthesis of 2,5‐dihydroxy[carboxy‐14C]benzoic acid (gentisic acid) is described. The procedure utilized [carboxy‐14C]salicylic acid as a starting material. Salicylic acid was converted to gentisic acid using potassium persulfate hydroxylation. The mixture of hydroxylation products in this reaction was easily separated by UV‐visualized quartz column chromatography to obtain the desired isomer in high purity (overall yield of 38%). This procedure may be used for micro and semimicro preparation of the title compound.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume23
Issue number3
DOIs
StatePublished - Mar 1986

Fingerprint

Hydroxylation
Benzoic Acid
Salicylic Acid
Column chromatography
Quartz
Isomers
Chromatography
Proteins
2,5-dihydroxybenzoic acid
potassium persulfate

Keywords

  • Carbon‐14
  • gentisic acid
  • hydroxylation
  • quartz column

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Drug Discovery
  • Spectroscopy
  • Organic Chemistry

Cite this

Synthesis of 2,5‐dihydroxy[carboxy‐14C]benzoic acid for electrochemical protein modification. / John, Varghese; Vennerstrom, Jonathan L.; Holmes, Thomas J.

In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 23, No. 3, 03.1986, p. 313-316.

Research output: Contribution to journalArticle

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