Synthesis and physical characterization of the self-complementary, alternating pyrimidine/purine hexanucleotide d[CGTACG]

Sandra Kuzmich, Luis A. Marky, Roger A. Jones

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The hexanucleotide d(CGTACG) has been synthesized by a phosphotriester method in which, for the first time, an 0-6 protected deoxyguanosine derivative has been used to avoid side reactions of the guanine residues. Conformational analysis by circular dichroism shows that d(CGTACG) maintains a B form under conditions (5 M NaCl) where the all C/G hexanucleotide d(CG)3 adopts a Z form, even though d(CGTACG) is a fully alternating pyrimidine/purine molecule. The ΔH for the helix-to-coil transition has been measured.

Original languageEnglish (US)
Pages (from-to)6265-6271
Number of pages7
JournalNucleic acids research
Issue number20
Publication statusPublished - Oct 25 1982


ASJC Scopus subject areas

  • Genetics

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