Synthesis and characterization of rat interleukin-10 (IL-10) cDNA clones from the RNA of cultured OX8- OX22- thoracic duct T cells

Richard E. Goodman, Jeb Oblak, Robin G. Bell

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Abstract

A cDNA of the complete coding region of rat IL-10 was cloned and sequenced using RNA isolated from a cultured population of thoracic duct T-lymphocytes obtained from Trichinella spiralis infected animals. The OX8- OX22-T-helper cells were stimulated in vitro with Concanavalin A for 24 hours prior to harvest. Reverse transcription of cellular RNA was primed with oligo-dT followed by amplification of IL-10 specific cDNA by polymerase chain reaction with synthetic oligo nucleotide primers chosen from two highly conserved regions of mouse and human IL-10. The sequence of the coding region of the amplified, cloned rat IL-10 cDNA is 90% identical to the mouse and 82% identical to the human IL-10 cDNA coding regions.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume189
Issue number1
DOIs
StatePublished - Nov 30 1992

Fingerprint

Thoracic Duct
T-cells
Interleukin-10
Ducts
Rats
Complementary DNA
Clone Cells
RNA
T-Lymphocytes
Trichinella spiralis
Polymerase chain reaction
Transcription
Concanavalin A
Helper-Inducer T-Lymphocytes
Reverse Transcription
Amplification
Animals
Nucleotides
Polymerase Chain Reaction
Population

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "A cDNA of the complete coding region of rat IL-10 was cloned and sequenced using RNA isolated from a cultured population of thoracic duct T-lymphocytes obtained from Trichinella spiralis infected animals. The OX8- OX22-T-helper cells were stimulated in vitro with Concanavalin A for 24 hours prior to harvest. Reverse transcription of cellular RNA was primed with oligo-dT followed by amplification of IL-10 specific cDNA by polymerase chain reaction with synthetic oligo nucleotide primers chosen from two highly conserved regions of mouse and human IL-10. The sequence of the coding region of the amplified, cloned rat IL-10 cDNA is 90{\%} identical to the mouse and 82{\%} identical to the human IL-10 cDNA coding regions.",
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