Syntaxin 3B is essential for the exocytosis of synaptic vesicles in ribbon synapses of the retina

L. Curtis, Proleta Datta, X. Liu, N. Bogdanova, R. Heidelberger, R. Janz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ribbon synapses of the vertebrate retina are specialized synapses that release neurotransmitter by synaptic vesicle exocytosis in a manner that is proportional to the level of depolarization of the cell. This release property is different from conventional neurons, in which the release of neurotransmitter occurs as a short-lived burst triggered by an action potential. Synaptic vesicle exocytosis is a calcium regulated process that is dependent on a set of interacting synaptic proteins that form the so-called SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) complex. Syntaxin 3B has been identified as a specialized SNARE molecule in ribbon synapses of the rodent retina. However, the best physiologically-characterized neuron that forms ribbon-style synapses is the rod-dominant or Mb1 bipolar cell of the goldfish retina. We report here the molecular characterization of syntaxin 3B from the goldfish retina. Using a combination of reverse transcription (RT) polymerase chain reaction (PCR) and immunostaining with a specific antibody, we show that syntaxin 3B is highly enriched in the plasma membrane of bipolar cell synaptic terminals of the goldfish retina. Using membrane capacitance measurements we demonstrate that a peptide derived from goldfish syntaxin 3B inhibits synaptic vesicle exocytosis. These experiments demonstrate that syntaxin 3B is an important factor for synaptic vesicle exocytosis in ribbon synapses of the vertebrate retina.

Original languageEnglish (US)
Pages (from-to)832-841
Number of pages10
JournalNeuroscience
Volume166
Issue number3
DOIs
StatePublished - Mar 31 2010

Fingerprint

Qa-SNARE Proteins
Synaptic Vesicles
Exocytosis
Synapses
Retina
Goldfish
SNARE Proteins
Neurotransmitter Agents
Vertebrates
Neurons
Presynaptic Terminals
Action Potentials
Reverse Transcription
Rodentia
Cell Membrane
Calcium
Polymerase Chain Reaction
Peptides
Membranes
Antibodies

Keywords

  • SNAP-25
  • SNARE
  • VAMP
  • splicing
  • synaptobrevin
  • vesicle fusion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Syntaxin 3B is essential for the exocytosis of synaptic vesicles in ribbon synapses of the retina. / Curtis, L.; Datta, Proleta; Liu, X.; Bogdanova, N.; Heidelberger, R.; Janz, R.

In: Neuroscience, Vol. 166, No. 3, 31.03.2010, p. 832-841.

Research output: Contribution to journalArticle

Curtis, L. ; Datta, Proleta ; Liu, X. ; Bogdanova, N. ; Heidelberger, R. ; Janz, R. / Syntaxin 3B is essential for the exocytosis of synaptic vesicles in ribbon synapses of the retina. In: Neuroscience. 2010 ; Vol. 166, No. 3. pp. 832-841.
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