Synergistic effect of local endothelial shear stress and systemic hypercholesterolemia on coronary atherosclerotic plaque progression and composition in pigs

Konstantinos C. Koskinas, Ioannis S Chatzizisis, Michail I. Papafaklis, Ahmet U. Coskun, Aaron B. Baker, Petr Jarolim, Antonios Antoniadis, Elazer R. Edelman, Peter H. Stone, Charles L. Feldman

Research output: Contribution to journalArticle

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Abstract

Background Systemic risk factors and local hemodynamic factors both contribute to coronary atherosclerosis, but their possibly synergistic inter-relationship remains unknown. The purpose of this natural history study was to investigate the combined in-vivo effect of varying levels of systemic hypercholesterolemia and local endothelial shear stress (ESS) on subsequent plaque progression and histological composition. Methods Diabetic, hyperlipidemic swine with higher systemic total cholesterol (TC) (n = 4) and relatively lower TC levels (n = 5) underwent three-vessel intravascular ultrasound (IVUS) at 3-5 consecutive time-points in-vivo. ESS was calculated serially using computational fluid dynamics. 3-D reconstructed coronary arteries were divided into 3 mm-long segments (n = 595), which were stratified according to higher vs. relatively lower TC and low (< 1.2 Pa) vs. higher local ESS (≥ 1.2 Pa). Arteries were harvested at 9 months, and a subset of segments (n = 114) underwent histopathologic analyses. Results Change of plaque volume (ΔPV) by IVUS over time was most pronounced in low-ESS segments from higher-TC animals. Notably, higher-ESS segments from higher-TC animals had greater ΔPV compared to low-ESS segments from lower-TC animals (p < 0.001). The time-averaged ESS in segments that resulted in significant plaque increased with increasing TC levels (slope: 0.24 Pa/100 mg/dl; r = 0.80; p < 0.01). At follow-up, low-ESS segments from higher-TC animals had the highest mRNA levels of lipoprotein receptors and inflammatory mediators and, consequently, the greatest lipid accumulation and inflammation. Conclusions This study redefines the principle concept that "low" ESS promotes coronary plaque growth and vulnerability by demonstrating that: (i.) the pro-atherogenic threshold of low ESS is not uniform, but cholesterol-dependent; and (ii.) the atherogenic effects of local low ESS are amplified, and the athero-protective effects of higher ESS may be outweighed, by increasing cholesterol levels. Intense hypercholesterolemia and very low ESS are synergistic in favoring rapid atheroma progression and high-risk composition.

Original languageEnglish (US)
Pages (from-to)394-401
Number of pages8
JournalInternational Journal of Cardiology
Volume169
Issue number6
DOIs
StatePublished - Nov 30 2013

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Atherosclerotic Plaques
Hypercholesterolemia
Swine
Cholesterol
Lipoprotein Receptors
Hydrodynamics
Natural History
Coronary Artery Disease
Coronary Vessels
Arteries
Hemodynamics
Inflammation
Lipids
Messenger RNA

Keywords

  • Coronary atherosclerosis
  • Endothelial shear stress
  • Histology
  • Hypercholesterolemia
  • Intravascular ultrasound

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Synergistic effect of local endothelial shear stress and systemic hypercholesterolemia on coronary atherosclerotic plaque progression and composition in pigs. / Koskinas, Konstantinos C.; Chatzizisis, Ioannis S; Papafaklis, Michail I.; Coskun, Ahmet U.; Baker, Aaron B.; Jarolim, Petr; Antoniadis, Antonios; Edelman, Elazer R.; Stone, Peter H.; Feldman, Charles L.

In: International Journal of Cardiology, Vol. 169, No. 6, 30.11.2013, p. 394-401.

Research output: Contribution to journalArticle

Koskinas, Konstantinos C. ; Chatzizisis, Ioannis S ; Papafaklis, Michail I. ; Coskun, Ahmet U. ; Baker, Aaron B. ; Jarolim, Petr ; Antoniadis, Antonios ; Edelman, Elazer R. ; Stone, Peter H. ; Feldman, Charles L. / Synergistic effect of local endothelial shear stress and systemic hypercholesterolemia on coronary atherosclerotic plaque progression and composition in pigs. In: International Journal of Cardiology. 2013 ; Vol. 169, No. 6. pp. 394-401.
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AU - Koskinas, Konstantinos C.

AU - Chatzizisis, Ioannis S

AU - Papafaklis, Michail I.

AU - Coskun, Ahmet U.

AU - Baker, Aaron B.

AU - Jarolim, Petr

AU - Antoniadis, Antonios

AU - Edelman, Elazer R.

AU - Stone, Peter H.

AU - Feldman, Charles L.

PY - 2013/11/30

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N2 - Background Systemic risk factors and local hemodynamic factors both contribute to coronary atherosclerosis, but their possibly synergistic inter-relationship remains unknown. The purpose of this natural history study was to investigate the combined in-vivo effect of varying levels of systemic hypercholesterolemia and local endothelial shear stress (ESS) on subsequent plaque progression and histological composition. Methods Diabetic, hyperlipidemic swine with higher systemic total cholesterol (TC) (n = 4) and relatively lower TC levels (n = 5) underwent three-vessel intravascular ultrasound (IVUS) at 3-5 consecutive time-points in-vivo. ESS was calculated serially using computational fluid dynamics. 3-D reconstructed coronary arteries were divided into 3 mm-long segments (n = 595), which were stratified according to higher vs. relatively lower TC and low (< 1.2 Pa) vs. higher local ESS (≥ 1.2 Pa). Arteries were harvested at 9 months, and a subset of segments (n = 114) underwent histopathologic analyses. Results Change of plaque volume (ΔPV) by IVUS over time was most pronounced in low-ESS segments from higher-TC animals. Notably, higher-ESS segments from higher-TC animals had greater ΔPV compared to low-ESS segments from lower-TC animals (p < 0.001). The time-averaged ESS in segments that resulted in significant plaque increased with increasing TC levels (slope: 0.24 Pa/100 mg/dl; r = 0.80; p < 0.01). At follow-up, low-ESS segments from higher-TC animals had the highest mRNA levels of lipoprotein receptors and inflammatory mediators and, consequently, the greatest lipid accumulation and inflammation. Conclusions This study redefines the principle concept that "low" ESS promotes coronary plaque growth and vulnerability by demonstrating that: (i.) the pro-atherogenic threshold of low ESS is not uniform, but cholesterol-dependent; and (ii.) the atherogenic effects of local low ESS are amplified, and the athero-protective effects of higher ESS may be outweighed, by increasing cholesterol levels. Intense hypercholesterolemia and very low ESS are synergistic in favoring rapid atheroma progression and high-risk composition.

AB - Background Systemic risk factors and local hemodynamic factors both contribute to coronary atherosclerosis, but their possibly synergistic inter-relationship remains unknown. The purpose of this natural history study was to investigate the combined in-vivo effect of varying levels of systemic hypercholesterolemia and local endothelial shear stress (ESS) on subsequent plaque progression and histological composition. Methods Diabetic, hyperlipidemic swine with higher systemic total cholesterol (TC) (n = 4) and relatively lower TC levels (n = 5) underwent three-vessel intravascular ultrasound (IVUS) at 3-5 consecutive time-points in-vivo. ESS was calculated serially using computational fluid dynamics. 3-D reconstructed coronary arteries were divided into 3 mm-long segments (n = 595), which were stratified according to higher vs. relatively lower TC and low (< 1.2 Pa) vs. higher local ESS (≥ 1.2 Pa). Arteries were harvested at 9 months, and a subset of segments (n = 114) underwent histopathologic analyses. Results Change of plaque volume (ΔPV) by IVUS over time was most pronounced in low-ESS segments from higher-TC animals. Notably, higher-ESS segments from higher-TC animals had greater ΔPV compared to low-ESS segments from lower-TC animals (p < 0.001). The time-averaged ESS in segments that resulted in significant plaque increased with increasing TC levels (slope: 0.24 Pa/100 mg/dl; r = 0.80; p < 0.01). At follow-up, low-ESS segments from higher-TC animals had the highest mRNA levels of lipoprotein receptors and inflammatory mediators and, consequently, the greatest lipid accumulation and inflammation. Conclusions This study redefines the principle concept that "low" ESS promotes coronary plaque growth and vulnerability by demonstrating that: (i.) the pro-atherogenic threshold of low ESS is not uniform, but cholesterol-dependent; and (ii.) the atherogenic effects of local low ESS are amplified, and the athero-protective effects of higher ESS may be outweighed, by increasing cholesterol levels. Intense hypercholesterolemia and very low ESS are synergistic in favoring rapid atheroma progression and high-risk composition.

KW - Coronary atherosclerosis

KW - Endothelial shear stress

KW - Histology

KW - Hypercholesterolemia

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