Synergism between human monocyte chemotactic and activating factor and bacterial products for activation of tumoricidal properties in murine macrophages

R. K. Singh, K. Berry, K. Matsushima, K. Yasumoto, I. J. Fidler

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Chemotactic factors regulate the recruitment of monocytes-macrophages to inflammatory sites and neoplastic tissues. The purpose of this study was to determine whether MCAF also influences the activation of C3H/HeN macrophages to become tumoricidal. Several metastatic and nonmetastatic clones of the K- 1735 murine melanoma cells syngeneic to C3H/HeN mice were transfected with expression vectors containing the human MCAF gene or control DNA. Tumor cells producing high levels of MCAF were significantly lysed by macrophages treated with LPS, whereas parental or control transfected cells were not. Control- activated macrophages incubated with both, IFN-γ and LPS lysed all the melanoma cells regardless of MCAF production. Pretreatment of macrophages with MCAF significantly enhanced their response to low concentrations of LPS, muramyl tripeptide, and a synthetic bacterial LPP, as measured by lysis of murine melanoma cells. These data suggest that in addition to being a chemotactic factor, MCAF can prime macrophages to respond to endotoxins and other bacterial products and therefore may regulate several levels of macrophage-tumor interactions in situ.

Original languageEnglish (US)
Pages (from-to)2786-2793
Number of pages8
JournalJournal of Immunology
Volume151
Issue number5
StatePublished - Jan 1 1993

Fingerprint

Chemokine CCL2
Macrophages
Melanoma
Chemotactic Factors
Inbred C3H Mouse
Endotoxins
Monocytes
Neoplasms
Clone Cells
DNA
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Synergism between human monocyte chemotactic and activating factor and bacterial products for activation of tumoricidal properties in murine macrophages. / Singh, R. K.; Berry, K.; Matsushima, K.; Yasumoto, K.; Fidler, I. J.

In: Journal of Immunology, Vol. 151, No. 5, 01.01.1993, p. 2786-2793.

Research output: Contribution to journalArticle

@article{52389997d7de442abeeb498879a072b4,
title = "Synergism between human monocyte chemotactic and activating factor and bacterial products for activation of tumoricidal properties in murine macrophages",
abstract = "Chemotactic factors regulate the recruitment of monocytes-macrophages to inflammatory sites and neoplastic tissues. The purpose of this study was to determine whether MCAF also influences the activation of C3H/HeN macrophages to become tumoricidal. Several metastatic and nonmetastatic clones of the K- 1735 murine melanoma cells syngeneic to C3H/HeN mice were transfected with expression vectors containing the human MCAF gene or control DNA. Tumor cells producing high levels of MCAF were significantly lysed by macrophages treated with LPS, whereas parental or control transfected cells were not. Control- activated macrophages incubated with both, IFN-γ and LPS lysed all the melanoma cells regardless of MCAF production. Pretreatment of macrophages with MCAF significantly enhanced their response to low concentrations of LPS, muramyl tripeptide, and a synthetic bacterial LPP, as measured by lysis of murine melanoma cells. These data suggest that in addition to being a chemotactic factor, MCAF can prime macrophages to respond to endotoxins and other bacterial products and therefore may regulate several levels of macrophage-tumor interactions in situ.",
author = "Singh, {R. K.} and K. Berry and K. Matsushima and K. Yasumoto and Fidler, {I. J.}",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "151",
pages = "2786--2793",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - Synergism between human monocyte chemotactic and activating factor and bacterial products for activation of tumoricidal properties in murine macrophages

AU - Singh, R. K.

AU - Berry, K.

AU - Matsushima, K.

AU - Yasumoto, K.

AU - Fidler, I. J.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Chemotactic factors regulate the recruitment of monocytes-macrophages to inflammatory sites and neoplastic tissues. The purpose of this study was to determine whether MCAF also influences the activation of C3H/HeN macrophages to become tumoricidal. Several metastatic and nonmetastatic clones of the K- 1735 murine melanoma cells syngeneic to C3H/HeN mice were transfected with expression vectors containing the human MCAF gene or control DNA. Tumor cells producing high levels of MCAF were significantly lysed by macrophages treated with LPS, whereas parental or control transfected cells were not. Control- activated macrophages incubated with both, IFN-γ and LPS lysed all the melanoma cells regardless of MCAF production. Pretreatment of macrophages with MCAF significantly enhanced their response to low concentrations of LPS, muramyl tripeptide, and a synthetic bacterial LPP, as measured by lysis of murine melanoma cells. These data suggest that in addition to being a chemotactic factor, MCAF can prime macrophages to respond to endotoxins and other bacterial products and therefore may regulate several levels of macrophage-tumor interactions in situ.

AB - Chemotactic factors regulate the recruitment of monocytes-macrophages to inflammatory sites and neoplastic tissues. The purpose of this study was to determine whether MCAF also influences the activation of C3H/HeN macrophages to become tumoricidal. Several metastatic and nonmetastatic clones of the K- 1735 murine melanoma cells syngeneic to C3H/HeN mice were transfected with expression vectors containing the human MCAF gene or control DNA. Tumor cells producing high levels of MCAF were significantly lysed by macrophages treated with LPS, whereas parental or control transfected cells were not. Control- activated macrophages incubated with both, IFN-γ and LPS lysed all the melanoma cells regardless of MCAF production. Pretreatment of macrophages with MCAF significantly enhanced their response to low concentrations of LPS, muramyl tripeptide, and a synthetic bacterial LPP, as measured by lysis of murine melanoma cells. These data suggest that in addition to being a chemotactic factor, MCAF can prime macrophages to respond to endotoxins and other bacterial products and therefore may regulate several levels of macrophage-tumor interactions in situ.

UR - http://www.scopus.com/inward/record.url?scp=0027236026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027236026&partnerID=8YFLogxK

M3 - Article

C2 - 8360492

AN - SCOPUS:0027236026

VL - 151

SP - 2786

EP - 2793

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -