Synchronization of drosophila cells in culture

Angie Rizzino, Alan B. Blumenthal

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalIn Vitro
Volume14
Issue number5
DOIs
StatePublished - May 1978

Fingerprint

Cell culture
Drosophila
Synchronization
cell culture
Cell Culture Techniques
Cells
S Phase
interphase
cells
cell lines
Cell Line
Culture Media
cell cycle
Cell Cycle
culture media
Growth
Population

Keywords

  • Drosophila
  • S phase
  • cell cycle
  • cell lines
  • cell synchrony

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Synchronization of drosophila cells in culture. / Rizzino, Angie; Blumenthal, Alan B.

In: In Vitro, Vol. 14, No. 5, 05.1978, p. 437-442.

Research output: Contribution to journalArticle

Rizzino, Angie ; Blumenthal, Alan B. / Synchronization of drosophila cells in culture. In: In Vitro. 1978 ; Vol. 14, No. 5. pp. 437-442.
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