Synaptotagmin-2 controls regulated exocytosis but not other secretory responses of mast cells

Ernestina Melicoff, Letecia Sansores-Garcia, Alejandra Gomez, Daniel C. Moreira, Proleta Datta, Pratima Thakur, Youlia Petrova, Tanya Siddiqi, Jayasimha N. Murthy, Burton F. Dickey, Ruth Heidelberger, Roberto Adachi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Mast cell degranulation is a highly regulated, calciumdependent process, which is important for the acute release of inflammatory mediators during the course of many pathological conditions. We previously found that Synaptotagmin-2, a calcium sensor in neuronal exocytosis, was expressed in a mast cell line. We postulated that this protein may be involved in the control of mast cell-regulated exocytosis, and we generated Synaptotagmin- 2 knock-out mice to test our hypothesis. Mast cells from this mutant animal conferred an abnormally decreased passive cutaneous anaphylaxis reaction on mast cell-deficient mice that correlated with a specific defect in mast cell-regulated exocytosis, leaving constitutive exocytosis and nonexocytic mast cell effector responses intact. This defect was not secondary to abnormalities in the development, maturation, migration, morphology, synthesis, and storage of inflammatory mediators, or intracellular calcium transients of the mast cells. Unlike neurons, the lack of Synaptotagmin-2 in mast cells was not associated with increased spontaneous exocytosis.

Original languageEnglish (US)
Pages (from-to)19445-19451
Number of pages7
JournalJournal of Biological Chemistry
Volume284
Issue number29
DOIs
StatePublished - Jul 17 2009

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Synaptotagmin II
Exocytosis
Mast Cells
Calcium
Defects
Neurons
Animals
Cells
Sensors
Cell Degranulation
Passive Cutaneous Anaphylaxis
Proteins
Knockout Mice

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Melicoff, E., Sansores-Garcia, L., Gomez, A., Moreira, D. C., Datta, P., Thakur, P., ... Adachi, R. (2009). Synaptotagmin-2 controls regulated exocytosis but not other secretory responses of mast cells. Journal of Biological Chemistry, 284(29), 19445-19451. https://doi.org/10.1074/jbc.M109.002550

Synaptotagmin-2 controls regulated exocytosis but not other secretory responses of mast cells. / Melicoff, Ernestina; Sansores-Garcia, Letecia; Gomez, Alejandra; Moreira, Daniel C.; Datta, Proleta; Thakur, Pratima; Petrova, Youlia; Siddiqi, Tanya; Murthy, Jayasimha N.; Dickey, Burton F.; Heidelberger, Ruth; Adachi, Roberto.

In: Journal of Biological Chemistry, Vol. 284, No. 29, 17.07.2009, p. 19445-19451.

Research output: Contribution to journalArticle

Melicoff, E, Sansores-Garcia, L, Gomez, A, Moreira, DC, Datta, P, Thakur, P, Petrova, Y, Siddiqi, T, Murthy, JN, Dickey, BF, Heidelberger, R & Adachi, R 2009, 'Synaptotagmin-2 controls regulated exocytosis but not other secretory responses of mast cells', Journal of Biological Chemistry, vol. 284, no. 29, pp. 19445-19451. https://doi.org/10.1074/jbc.M109.002550
Melicoff, Ernestina ; Sansores-Garcia, Letecia ; Gomez, Alejandra ; Moreira, Daniel C. ; Datta, Proleta ; Thakur, Pratima ; Petrova, Youlia ; Siddiqi, Tanya ; Murthy, Jayasimha N. ; Dickey, Burton F. ; Heidelberger, Ruth ; Adachi, Roberto. / Synaptotagmin-2 controls regulated exocytosis but not other secretory responses of mast cells. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 29. pp. 19445-19451.
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