SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production

Zhiye Wang, Zeyang Ma, Claudia Castillo-González, Di Sun, Yanjun Li, Bin Yu, Baoyu Zhao, Pingwei Li, Xiuren Zhang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chromatin remodelling factors (CHRs) typically function to alter chromatin structure. CHRs also reside in ribonucleoprotein complexes, but little is known about their RNA-related functions. Here we show that CHR2 (also known as BRM), the ATPase subunit of the large switch/sucrose non-fermentable (SWI/SNF) complex, is a partner of the Microprocessor component Serrate (SE). CHR2 promotes the transcription of primary microRNA precursors (pri-miRNAs) while repressing miRNA accumulation in vivo. Direct interaction with SE is required for post-transcriptional inhibition of miRNA accumulation by CHR2 but not for its transcriptional activity. CHR2 can directly bind to and unwind pri-miRNAs and inhibit their processing, and this inhibition requires the remodelling and helicase activity of CHR2 in vitro and in vivo. Furthermore, the secondary structures of pri-miRNAs differed between wild-type Arabidopsis thaliana and chr2 mutants. We conclude that CHR2 accesses pri-miRNAs through SE and remodels their secondary structures, preventing downstream processing by DCL1 and HYL1. Our study uncovers pri-miRNAs as a substrate of CHR2, and an additional regulatory layer upstream of Microprocessor activity to control miRNA accumulation.

Original languageEnglish (US)
Pages (from-to)516-521
Number of pages6
JournalNature
Volume557
Issue number7706
DOIs
StatePublished - May 24 2018

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MicroRNAs
Adenosine Triphosphatases
Chromatin Assembly and Disassembly
Microcomputers
Ribonucleoproteins
Arabidopsis
Chromatin
Sucrose
RNA

ASJC Scopus subject areas

  • General

Cite this

Wang, Z., Ma, Z., Castillo-González, C., Sun, D., Li, Y., Yu, B., ... Zhang, X. (2018). SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production. Nature, 557(7706), 516-521. https://doi.org/10.1038/s41586-018-0135-x

SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production. / Wang, Zhiye; Ma, Zeyang; Castillo-González, Claudia; Sun, Di; Li, Yanjun; Yu, Bin; Zhao, Baoyu; Li, Pingwei; Zhang, Xiuren.

In: Nature, Vol. 557, No. 7706, 24.05.2018, p. 516-521.

Research output: Contribution to journalArticle

Wang, Z, Ma, Z, Castillo-González, C, Sun, D, Li, Y, Yu, B, Zhao, B, Li, P & Zhang, X 2018, 'SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production', Nature, vol. 557, no. 7706, pp. 516-521. https://doi.org/10.1038/s41586-018-0135-x
Wang Z, Ma Z, Castillo-González C, Sun D, Li Y, Yu B et al. SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production. Nature. 2018 May 24;557(7706):516-521. https://doi.org/10.1038/s41586-018-0135-x
Wang, Zhiye ; Ma, Zeyang ; Castillo-González, Claudia ; Sun, Di ; Li, Yanjun ; Yu, Bin ; Zhao, Baoyu ; Li, Pingwei ; Zhang, Xiuren. / SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production. In: Nature. 2018 ; Vol. 557, No. 7706. pp. 516-521.
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