Survey of porcine rotavirus G and P genotype in Poland and the United States using RT-PCR

S. Winiarczyk, P. S. Paul, S. Mummidi, R. Panek, Z. Gradzki

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Porcine rotaviruses are a common cause of gastroenteritis. Several serotypes have been detected based on the two surface proteins VP4 (P-types) and VP7 (G-types). However, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. In this study, we describe a reverse transcriptase-polymerase chain reaction (RT-PCR) method for the typing of P and G types of rotavirus. This method allowed to detect G and P types in 96.8 and 87.1% of isolates collected in the United States, respectively and in 54.5 and 38.6% of isolates collected in Poland, respectively. Within the US specimens the G3, G4, G5, G9 and G10 types were detected in combination with P6 and P7 types while among Polish specimens only G3, G4 and G5 types in combination with P6 and P7 types were identified. In both instances the G4 and G5 were the most prevalent types. These studies show that a RT-PCR typing method is suitable for molecular epidemiological studies and that there is more diversity among porcine rotavirus than previously reported.

Original languageEnglish (US)
Pages (from-to)373-378
Number of pages6
JournalJournal of Veterinary Medicine, Series B
Volume49
Issue number8
DOIs
StatePublished - Oct 1 2002

Fingerprint

Rotavirus
Poland
Reverse Transcriptase Polymerase Chain Reaction
Swine
reverse transcriptase polymerase chain reaction
Genotype
swine
genotype
gastroenteritis
surface proteins
Gastroenteritis
methodology
epidemiological studies
Epidemiologic Studies
Membrane Proteins
serotypes
Surveys and Questionnaires

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Survey of porcine rotavirus G and P genotype in Poland and the United States using RT-PCR. / Winiarczyk, S.; Paul, P. S.; Mummidi, S.; Panek, R.; Gradzki, Z.

In: Journal of Veterinary Medicine, Series B, Vol. 49, No. 8, 01.10.2002, p. 373-378.

Research output: Contribution to journalArticle

Winiarczyk, S. ; Paul, P. S. ; Mummidi, S. ; Panek, R. ; Gradzki, Z. / Survey of porcine rotavirus G and P genotype in Poland and the United States using RT-PCR. In: Journal of Veterinary Medicine, Series B. 2002 ; Vol. 49, No. 8. pp. 373-378.
@article{ea709fbc3d8a436eb21b308c80fef6d3,
title = "Survey of porcine rotavirus G and P genotype in Poland and the United States using RT-PCR",
abstract = "Porcine rotaviruses are a common cause of gastroenteritis. Several serotypes have been detected based on the two surface proteins VP4 (P-types) and VP7 (G-types). However, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. In this study, we describe a reverse transcriptase-polymerase chain reaction (RT-PCR) method for the typing of P and G types of rotavirus. This method allowed to detect G and P types in 96.8 and 87.1{\%} of isolates collected in the United States, respectively and in 54.5 and 38.6{\%} of isolates collected in Poland, respectively. Within the US specimens the G3, G4, G5, G9 and G10 types were detected in combination with P6 and P7 types while among Polish specimens only G3, G4 and G5 types in combination with P6 and P7 types were identified. In both instances the G4 and G5 were the most prevalent types. These studies show that a RT-PCR typing method is suitable for molecular epidemiological studies and that there is more diversity among porcine rotavirus than previously reported.",
author = "S. Winiarczyk and Paul, {P. S.} and S. Mummidi and R. Panek and Z. Gradzki",
year = "2002",
month = "10",
day = "1",
doi = "10.1046/j.1439-0450.2002.00572.x",
language = "English (US)",
volume = "49",
pages = "373--378",
journal = "Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health",
issn = "0931-1793",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "8",

}

TY - JOUR

T1 - Survey of porcine rotavirus G and P genotype in Poland and the United States using RT-PCR

AU - Winiarczyk, S.

AU - Paul, P. S.

AU - Mummidi, S.

AU - Panek, R.

AU - Gradzki, Z.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Porcine rotaviruses are a common cause of gastroenteritis. Several serotypes have been detected based on the two surface proteins VP4 (P-types) and VP7 (G-types). However, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. In this study, we describe a reverse transcriptase-polymerase chain reaction (RT-PCR) method for the typing of P and G types of rotavirus. This method allowed to detect G and P types in 96.8 and 87.1% of isolates collected in the United States, respectively and in 54.5 and 38.6% of isolates collected in Poland, respectively. Within the US specimens the G3, G4, G5, G9 and G10 types were detected in combination with P6 and P7 types while among Polish specimens only G3, G4 and G5 types in combination with P6 and P7 types were identified. In both instances the G4 and G5 were the most prevalent types. These studies show that a RT-PCR typing method is suitable for molecular epidemiological studies and that there is more diversity among porcine rotavirus than previously reported.

AB - Porcine rotaviruses are a common cause of gastroenteritis. Several serotypes have been detected based on the two surface proteins VP4 (P-types) and VP7 (G-types). However, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. In this study, we describe a reverse transcriptase-polymerase chain reaction (RT-PCR) method for the typing of P and G types of rotavirus. This method allowed to detect G and P types in 96.8 and 87.1% of isolates collected in the United States, respectively and in 54.5 and 38.6% of isolates collected in Poland, respectively. Within the US specimens the G3, G4, G5, G9 and G10 types were detected in combination with P6 and P7 types while among Polish specimens only G3, G4 and G5 types in combination with P6 and P7 types were identified. In both instances the G4 and G5 were the most prevalent types. These studies show that a RT-PCR typing method is suitable for molecular epidemiological studies and that there is more diversity among porcine rotavirus than previously reported.

UR - http://www.scopus.com/inward/record.url?scp=0036807158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036807158&partnerID=8YFLogxK

U2 - 10.1046/j.1439-0450.2002.00572.x

DO - 10.1046/j.1439-0450.2002.00572.x

M3 - Article

C2 - 12449245

AN - SCOPUS:0036807158

VL - 49

SP - 373

EP - 378

JO - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

JF - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

SN - 0931-1793

IS - 8

ER -