Surfactant proteins A and D enhance the phagocytosis of Chlamydia into THP-1 cells

Rebecca E. Oberley, Kevin A. Ault, Traci L. Neff, Kavita R. Khubchandani, Erika C. Crouch, Jeanne M. Snyder

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Chlamydiae are intracellular bacterial pathogens that infect mucosal surfaces, i.e., the epithelium of the lung, genital tract, and conjunctiva of the eye, as well as alveolar macrophages. In the present study, we show that pulmonary surfactant protein A (SP-A) and surfactant protein D (SP-D), lung collectins involved in innate host defense, enhance the phagocytosis of Chlamydia pneumoniae and Chlamydia trachomatis by THP-1 cells, a human monocyte/macrophage cell line. We also show that SP-A is able to aggregate both C. trachomatis and C. pneumoniae but that SP-D only aggregates C. pneumoniae. In addition, we found that after phagocytosis in the presence of SP-A, the number of viable C. trachomatis pathogens in the THP-1 cells 48 h later was increased ∼3.5-fold. These findings suggest that SP-A and SP-D interact with chlamydial pathogens and enhance their phagocytosis into macrophages. In addition, the chlamydial pathogens internalized in the presence of collectins are able to grow and replicate in the THP-1 cells after phagocytosis.

Original languageEnglish (US)
Pages (from-to)L296-L306
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume287
Issue number2 31-2
DOIs
StatePublished - Aug 1 2004

Fingerprint

Pulmonary Surfactant-Associated Protein D
Pulmonary Surfactant-Associated Protein A
Chlamydia
Phagocytosis
Chlamydophila pneumoniae
Chlamydia trachomatis
Collectins
Pulmonary Surfactant-Associated Proteins
Macrophages
Cytophagocytosis
Lung
Conjunctiva
Alveolar Macrophages
Monocytes
Epithelium
Cell Line

Keywords

  • Chlamydia pneumoniae
  • Chlamydia trachomatis
  • SP-A
  • SP-D

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Surfactant proteins A and D enhance the phagocytosis of Chlamydia into THP-1 cells. / Oberley, Rebecca E.; Ault, Kevin A.; Neff, Traci L.; Khubchandani, Kavita R.; Crouch, Erika C.; Snyder, Jeanne M.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 287, No. 2 31-2, 01.08.2004, p. L296-L306.

Research output: Contribution to journalArticle

Oberley, Rebecca E. ; Ault, Kevin A. ; Neff, Traci L. ; Khubchandani, Kavita R. ; Crouch, Erika C. ; Snyder, Jeanne M. / Surfactant proteins A and D enhance the phagocytosis of Chlamydia into THP-1 cells. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2004 ; Vol. 287, No. 2 31-2. pp. L296-L306.
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