Surfactant protein D is present in the human female reproductive tract and inhibits Chlamydia trachomatis infection

Rebecca E Deegan, Kelli L. Goss, Kevin A. Ault, Erika C. Crouch, Jeanne M. Snyder

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Surfactant protein D (SP-D) is a lung collectin involved in innate host defence mechanisms in the lung. SP-D is also expressed at other mucosal sites throughout the human body. In the present study, we show that SP-D mRNA and protein are expressed in the human female reproductive tract. SP-D protein was localized in the apical portion of the reproductive epithelial cells. We also demonstrate that endometrial and endocervical cell lines and primary endocervical cells in culture produce SP-D mRNA and protein. Chlamydia trachomatis is an intracellular pathogen that infects the female reproductive tract, primarily the cervix, and is responsible for the most prevalent infectious disease in the USA. Untreated chlamydial infections of the female reproductive tract often result in sterility of the infected woman. Since SP-D protein is produced in cervical glands, we examined the effect of SP-D on chlamydial infection of cervical epithelial cells in vitro. We found that SP-D protein inhibits the infection of HeLa cells (an endocervical epithelial cell line) by C. trachomatis in a dose-dependent manner. We further demonstrate that the SP-D lectin-binding domain is involved in inhibiting infection of HeLa cells by Chlamydia. In conclusion, we detected SP-D in the female reproductive tract and determined that one of the functions of the SP-D protein may be to protect cervical epithelial cells from infection by C. trachomatis.

Original languageEnglish (US)
Pages (from-to)861-870
Number of pages10
JournalMolecular Human Reproduction
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2004

Fingerprint

Pulmonary Surfactant-Associated Protein D
Chlamydia Infections
Chlamydia trachomatis
Epithelial Cells
Proteins
Infection
HeLa Cells
Collectins
Reproductive Tract Infections
Cell Line
Lung
Messenger RNA
Chlamydia
Human Body
Lectins
Cervix Uteri
Infertility
Communicable Diseases

Keywords

  • Chlamydia trachomatis
  • Human
  • Innate host defence
  • Reproductive tract
  • Surfactant protein D

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Cell Biology

Cite this

Surfactant protein D is present in the human female reproductive tract and inhibits Chlamydia trachomatis infection. / Deegan, Rebecca E; Goss, Kelli L.; Ault, Kevin A.; Crouch, Erika C.; Snyder, Jeanne M.

In: Molecular Human Reproduction, Vol. 10, No. 12, 01.12.2004, p. 861-870.

Research output: Contribution to journalArticle

Deegan, Rebecca E ; Goss, Kelli L. ; Ault, Kevin A. ; Crouch, Erika C. ; Snyder, Jeanne M. / Surfactant protein D is present in the human female reproductive tract and inhibits Chlamydia trachomatis infection. In: Molecular Human Reproduction. 2004 ; Vol. 10, No. 12. pp. 861-870.
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