Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

Angela K. Pannier, Julie A. Wieland, Lonnie D. Shea

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG- and carboxylic acid-terminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.

Original languageEnglish (US)
Pages (from-to)26-39
Number of pages14
JournalActa Biomaterialia
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2008

Fingerprint

Ethylene Glycol
Self assembled monolayers
Ethylene glycol
Polyethylene glycols
Genes
Transfection
Substrates
Surface chemistry
Immobilization
Gene transfer
Gene therapy
Atomic Force Microscopy
Cell adhesion
Biocompatible Materials
Tissue Engineering
Scaffolds (biology)
Carboxylic Acids
Carboxylic acids
Tissue engineering
Biomaterials

Keywords

  • Atomic force microscopy (AFM)
  • Gene delivery
  • Reverse transfection
  • Self-assembled monolayers

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes. / Pannier, Angela K.; Wieland, Julie A.; Shea, Lonnie D.

In: Acta Biomaterialia, Vol. 4, No. 1, 01.01.2008, p. 26-39.

Research output: Contribution to journalArticle

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