Surface modification of chitosan for selective surface-protein interaction

Anuradha Subramanian, Anand V. Rau, Hemanth Kaligotla

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study evaluates the chromatographic performance of a support obtained by the reaction of a variety of ligands with di-epoxide (1,4 butanediol diglycidyl ether) activated chitosan beads. Chitosan beads 400-625 microns (μm) in diameter and with a solid contents of 4.5% were selected for this study. The activation step was optimized with respect to time, temperature, and starting epoxide concentration. Epoxide activity in the range of 600-1200 μmoles of epoxide per gram of chitosan was obtained. Epoxide activated beads were then reacted with synthetic ligands to yield a support for use in bioseparations. Ligand modified chitosan beads were observed to selectively bind human serum albumin (HSA) over human fibrinogen and human immunoglobulin. Purity of the products, obtained from a single purification step, as judged by electrophoretic analysis was estimated to be greater than 95%.

Original languageEnglish (US)
Pages (from-to)321-332
Number of pages12
JournalCarbohydrate Polymers
Volume66
Issue number3
DOIs
StatePublished - Nov 2 2006

Fingerprint

Epoxy Compounds
surface proteins
Chitosan
chitosan
epoxides
Surface treatment
Membrane Proteins
Proteins
Ligands
butanediol
Purification
fibrinogen
Ethers
Serum Albumin
purity
Chemical activation
Fibrinogen
immunoglobulins
Immunoglobulins
ethers

Keywords

  • Albumin binding
  • Chemical modification
  • Chitosan

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Surface modification of chitosan for selective surface-protein interaction. / Subramanian, Anuradha; Rau, Anand V.; Kaligotla, Hemanth.

In: Carbohydrate Polymers, Vol. 66, No. 3, 02.11.2006, p. 321-332.

Research output: Contribution to journalArticle

Subramanian, Anuradha ; Rau, Anand V. ; Kaligotla, Hemanth. / Surface modification of chitosan for selective surface-protein interaction. In: Carbohydrate Polymers. 2006 ; Vol. 66, No. 3. pp. 321-332.
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