Surface display of recombinant proteins on Bacillus thuringiensis spores

Cheng Du, Wing C. Chan, Timothy W. McKeithan, Kenneth W. Nickerson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.

Original languageEnglish (US)
Pages (from-to)3337-3341
Number of pages5
JournalApplied and environmental microbiology
Volume71
Issue number6
DOIs
StatePublished - Jun 1 2005

Fingerprint

Bacillus thuringiensis
Spores
Recombinant Proteins
recombinant proteins
antibody
spore
spores
antibodies
protein
Single-Chain Antibodies
sporulation
Antibodies
Green Fluorescent Proteins
green fluorescent protein
promoter regions
gene
Genes
Proteins
genes
proteins

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Surface display of recombinant proteins on Bacillus thuringiensis spores. / Du, Cheng; Chan, Wing C.; McKeithan, Timothy W.; Nickerson, Kenneth W.

In: Applied and environmental microbiology, Vol. 71, No. 6, 01.06.2005, p. 3337-3341.

Research output: Contribution to journalArticle

Du, Cheng ; Chan, Wing C. ; McKeithan, Timothy W. ; Nickerson, Kenneth W. / Surface display of recombinant proteins on Bacillus thuringiensis spores. In: Applied and environmental microbiology. 2005 ; Vol. 71, No. 6. pp. 3337-3341.
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