Supraceliac, but not infrarenal, aortic cross-clamping upregulates neutrophil integrin CD11b

Gary E. Hill, Paul J. Mihalakakos, John R. Spurzem, Bernard Timothy Baxter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To evaluate the effects of supraceliac and infrarenal aortic cross-clamping on the expression of neutrophil integrin CD11b (a marker of systemic cytokine release). Design: Two groups, determined by anatomic placement of aortic cross-clamp. Laboratory personnel were blinded as to group assignment. Setting: University teaching and community hospitals. Laboratory facilities used were university and Veteran's Affairs medical centers. Participants: Patients scheduled for aortic surgery. Interventions: Blood sampling was performed at baseline, after 30 minutes of aortic cross-clamp duration, 30 and 90 minutes after reperfusion (for tumor necrosis factor-α plasma levels in infrarenal cross-clamp group), and at baseline and 90 minutes reperfusion (for neutrophil CD11b expression quantification) in both groups. Measurements and Main Results: Tumor necrosis factor-α measured by ELISA technique did not change at any time period in the infrarenal clamping group. Neutrophil CD11b expression, measured by double antibody staining and FACScan analysis, did not change significantly at 90 minutes of reperfusion in the infrarenal group, but increased significantly (p < 0.05) in the supraceliac aortic cross-clamp group. Conclusions: Neutrophil integrin CD11b has been demonstrated to be the primary adhesive glycoprotein responsible for neutrophil organ entrapment and subsequent neutrophil-mediated reperfusion injury. These results suggest that upregulation of neutrophil integrin CD11b after supraceliac aortic clamping may in part be responsible for the higher incidence of acute lung injury after thoracic aortic aneurysm repair requiring supraceliac clamping when compared with infrarenal aneurysm surgery.

Original languageEnglish (US)
Pages (from-to)515-518
Number of pages4
JournalJournal of Cardiothoracic and Vascular Anesthesia
Volume9
Issue number5
DOIs
StatePublished - Oct 1995

Fingerprint

Constriction
Integrins
Neutrophils
Up-Regulation
Reperfusion
Tumor Necrosis Factor-alpha
Laboratory Personnel
Thoracic Aortic Aneurysm
Acute Lung Injury
Community Hospital
Veterans
Reperfusion Injury
Teaching Hospitals
Adhesives
Aneurysm
Glycoproteins
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Cytokines
Antibodies

Keywords

  • aortic cross-clamping
  • aortic surgery
  • cytokines

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Anesthesiology and Pain Medicine

Cite this

Supraceliac, but not infrarenal, aortic cross-clamping upregulates neutrophil integrin CD11b. / Hill, Gary E.; Mihalakakos, Paul J.; Spurzem, John R.; Baxter, Bernard Timothy.

In: Journal of Cardiothoracic and Vascular Anesthesia, Vol. 9, No. 5, 10.1995, p. 515-518.

Research output: Contribution to journalArticle

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abstract = "Objective: To evaluate the effects of supraceliac and infrarenal aortic cross-clamping on the expression of neutrophil integrin CD11b (a marker of systemic cytokine release). Design: Two groups, determined by anatomic placement of aortic cross-clamp. Laboratory personnel were blinded as to group assignment. Setting: University teaching and community hospitals. Laboratory facilities used were university and Veteran's Affairs medical centers. Participants: Patients scheduled for aortic surgery. Interventions: Blood sampling was performed at baseline, after 30 minutes of aortic cross-clamp duration, 30 and 90 minutes after reperfusion (for tumor necrosis factor-α plasma levels in infrarenal cross-clamp group), and at baseline and 90 minutes reperfusion (for neutrophil CD11b expression quantification) in both groups. Measurements and Main Results: Tumor necrosis factor-α measured by ELISA technique did not change at any time period in the infrarenal clamping group. Neutrophil CD11b expression, measured by double antibody staining and FACScan analysis, did not change significantly at 90 minutes of reperfusion in the infrarenal group, but increased significantly (p < 0.05) in the supraceliac aortic cross-clamp group. Conclusions: Neutrophil integrin CD11b has been demonstrated to be the primary adhesive glycoprotein responsible for neutrophil organ entrapment and subsequent neutrophil-mediated reperfusion injury. These results suggest that upregulation of neutrophil integrin CD11b after supraceliac aortic clamping may in part be responsible for the higher incidence of acute lung injury after thoracic aortic aneurysm repair requiring supraceliac clamping when compared with infrarenal aneurysm surgery.",
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