Suppressor cell activation by anti-Thy 1.2 in NZB/WC1 mice

Lynell Warren Klassen, A. Zarate, M. C. Gelfand, A. D. Steinberg

Research output: Contribution to journalArticle

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Abstract

A single injection of anti-Thy 1.2 serum was used to study the spontaneous loss of suppressor cells in NZB/WF1 mice. When given 1 day before primary allografting with C57BL/6 skin, anti-Thy 1.2 significantly prolonged mean graft survival (MGS) in 5-week-old NZB/NZWF1 graft recipients. However, four times the dose of anti-Thy 1.2 was required to cause graft prolongation when compared with BALB/c recipients. In contrast to its graft-prolonging effect in 5-week NZB/WF1 mice, anti-Thy 1.2 failed to prolong MGS in either 17- or 34-week-old NZB/WF1 animals. Spleen cells from anti-Thy 1.2-treated 5-week-old NZB/WF1 mice could transfer suppression of graft rejection to otherwise untreated 5-week-old syngeneic recipients. This transfer required viable, radioresistant T cells. Although spleen cells from anti-Thy 1.2-treated 5-week-old mice transferred the suppression to 5-week-old recipients, the same cells were not effective if given to 17-week-old recipients. However, spleen cells from 17-week-old-pretreated donor mice were able to prolong MGS when given to the younger 5-week-old syngeneic recipients. The transfer studies of skin allograft prolongation by anti-Thy 1.2-treated spleen cells suggest that the ability to initiate suppressor cell activation is present in both young and older NZB/WF1 mice, whereas suppressor effector mechanisms are deficient in the older mice. In addition to its usefulness in studying the details of suppressor cell action, the studies suggest that anti-Thy 1.2 treatment may be a valuable probe for the presence of suppressor cells and/or their precursors.

Original languageEnglish (US)
Pages (from-to)2067-2072
Number of pages6
JournalJournal of Immunology
Volume119
Issue number6
StatePublished - Dec 1 1977

Fingerprint

Inbred NZB Mouse
Graft Survival
Spleen
Transplants
Skin
Homologous Transplantation
Graft Rejection
Allografts
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Klassen, L. W., Zarate, A., Gelfand, M. C., & Steinberg, A. D. (1977). Suppressor cell activation by anti-Thy 1.2 in NZB/WC1 mice. Journal of Immunology, 119(6), 2067-2072.

Suppressor cell activation by anti-Thy 1.2 in NZB/WC1 mice. / Klassen, Lynell Warren; Zarate, A.; Gelfand, M. C.; Steinberg, A. D.

In: Journal of Immunology, Vol. 119, No. 6, 01.12.1977, p. 2067-2072.

Research output: Contribution to journalArticle

Klassen, LW, Zarate, A, Gelfand, MC & Steinberg, AD 1977, 'Suppressor cell activation by anti-Thy 1.2 in NZB/WC1 mice', Journal of Immunology, vol. 119, no. 6, pp. 2067-2072.
Klassen LW, Zarate A, Gelfand MC, Steinberg AD. Suppressor cell activation by anti-Thy 1.2 in NZB/WC1 mice. Journal of Immunology. 1977 Dec 1;119(6):2067-2072.
Klassen, Lynell Warren ; Zarate, A. ; Gelfand, M. C. ; Steinberg, A. D. / Suppressor cell activation by anti-Thy 1.2 in NZB/WC1 mice. In: Journal of Immunology. 1977 ; Vol. 119, No. 6. pp. 2067-2072.
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