Suppression of tumor-forming ability and related traits in MCF-7 human breast cancer cells by fusion with immortal mammary epithelial cells

Deborah A. Zajchowski, Vimla Band, Douglas K. Trask, David Kling, James L. Connolly, Ruth Sager

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Somatic cell hybrids between MCF-7 human breast cancer cells and normal immortalized human mammary epithelial cells have been obtained by polyethylene glycol-mediated cell fusion. The hybrid cells are suppressed in their ability to form tumors in nude mice, as well as in traits specific to the tumorigenic MCF-7 parent: growth factor independence, tumor necrosis factor sensitivity, and pS2 gene expression. In addition, they display other characteristics of the "normal" parent, including increased expression relative to the MCF-7 cells of the genes for the extracellular matrix component fibronectin, the intermediate filament keratin 5, and the angiogenesis inhibitor thrombospondin. The levels of keratins 8 and 18 also resemble those of the nontumorigenic parent. These results provide evidence for the existence of tumor suppressor gene products in immortal mammary epithelial cells. We propose a characteristic "suppressed" tumor cell phenotype, which encompasses altered cytoarchitecture, angiogenesis capabilities, and growth factor requirements.

Original languageEnglish (US)
Pages (from-to)2314-2318
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number6
DOIs
StatePublished - Jan 1 1990

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Cell Fusion
Hybrid Cells
Intercellular Signaling Peptides and Proteins
Breast
Epithelial Cells
Keratin-5
Keratin-8
Breast Neoplasms
Keratin-18
Thrombospondins
Angiogenesis Inhibitors
Intermediate Filaments
Angiogenesis Inducing Agents
MCF-7 Cells
Tumor Suppressor Genes
Fibronectins
Nude Mice
Extracellular Matrix
Neoplasms
Tumor Necrosis Factor-alpha

Keywords

  • Fibronectin
  • Keratins
  • Somatic cell hybrids
  • Thrombospondin
  • Tumor suppression

ASJC Scopus subject areas

  • General

Cite this

Suppression of tumor-forming ability and related traits in MCF-7 human breast cancer cells by fusion with immortal mammary epithelial cells. / Zajchowski, Deborah A.; Band, Vimla; Trask, Douglas K.; Kling, David; Connolly, James L.; Sager, Ruth.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 6, 01.01.1990, p. 2314-2318.

Research output: Contribution to journalArticle

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