Suppression of transgene silencing by matrix attachment regions in maize

A dual role for the maize 5′ ADH1 matrix attachment region

Cory Brouwer, Wesley Bruce, Sheila Maddock, Zoya V Avramova, Ben Bowen

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Matrix attachment regions (MARs) are DNA sequences that bind an internal nuclear network of nonhistone proteins called the nuclear matrix. Thus, they may define discrete gene-containing chromatin loops in vivo. We have studied the effects of flanking transgenes with MARs on transgene expression levels in maize callus and in transformed maize plants. Three MAR elements, two from maize (Adh1 5′ MAR and Mha1 5′ MAR) and one from yeast (ARS1), had very different effects on transgene expression that bore no relation to their affinity for the nuclear matrix in vitro. In callus, two of the MAR elements (Adh1 5′ MAR and ARS1) reduced transgene silencing but had no effect on the variability of expression. In transgenic plants, Adh1 5′ MAR had the effect of localizing β-glucuronidase expression to lateral root initiation sites. A possible model accounting for the function of Adh1 5′ MAR is discussed.

Original languageEnglish (US)
Pages (from-to)2251-2264
Number of pages14
JournalPlant Cell
Volume14
Issue number9
DOIs
StatePublished - Sep 1 2002

Fingerprint

Matrix Attachment Regions
Transgenes
transgenes
Zea mays
nuclear matrix
corn
callus
Bony Callus
chromatin
transgenic plants
yeasts
Nuclear Matrix-Associated Proteins
nucleotide sequences
Nuclear Matrix
Genetically Modified Plants
DNA sequences
Glucuronidase
Yeast
Chromatin
genes

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Suppression of transgene silencing by matrix attachment regions in maize : A dual role for the maize 5′ ADH1 matrix attachment region. / Brouwer, Cory; Bruce, Wesley; Maddock, Sheila; Avramova, Zoya V; Bowen, Ben.

In: Plant Cell, Vol. 14, No. 9, 01.09.2002, p. 2251-2264.

Research output: Contribution to journalArticle

@article{2cdab0004b5a451a8897cc7843cb7f05,
title = "Suppression of transgene silencing by matrix attachment regions in maize: A dual role for the maize 5′ ADH1 matrix attachment region",
abstract = "Matrix attachment regions (MARs) are DNA sequences that bind an internal nuclear network of nonhistone proteins called the nuclear matrix. Thus, they may define discrete gene-containing chromatin loops in vivo. We have studied the effects of flanking transgenes with MARs on transgene expression levels in maize callus and in transformed maize plants. Three MAR elements, two from maize (Adh1 5′ MAR and Mha1 5′ MAR) and one from yeast (ARS1), had very different effects on transgene expression that bore no relation to their affinity for the nuclear matrix in vitro. In callus, two of the MAR elements (Adh1 5′ MAR and ARS1) reduced transgene silencing but had no effect on the variability of expression. In transgenic plants, Adh1 5′ MAR had the effect of localizing β-glucuronidase expression to lateral root initiation sites. A possible model accounting for the function of Adh1 5′ MAR is discussed.",
author = "Cory Brouwer and Wesley Bruce and Sheila Maddock and Avramova, {Zoya V} and Ben Bowen",
year = "2002",
month = "9",
day = "1",
doi = "10.1105/tpc.004028",
language = "English (US)",
volume = "14",
pages = "2251--2264",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "9",

}

TY - JOUR

T1 - Suppression of transgene silencing by matrix attachment regions in maize

T2 - A dual role for the maize 5′ ADH1 matrix attachment region

AU - Brouwer, Cory

AU - Bruce, Wesley

AU - Maddock, Sheila

AU - Avramova, Zoya V

AU - Bowen, Ben

PY - 2002/9/1

Y1 - 2002/9/1

N2 - Matrix attachment regions (MARs) are DNA sequences that bind an internal nuclear network of nonhistone proteins called the nuclear matrix. Thus, they may define discrete gene-containing chromatin loops in vivo. We have studied the effects of flanking transgenes with MARs on transgene expression levels in maize callus and in transformed maize plants. Three MAR elements, two from maize (Adh1 5′ MAR and Mha1 5′ MAR) and one from yeast (ARS1), had very different effects on transgene expression that bore no relation to their affinity for the nuclear matrix in vitro. In callus, two of the MAR elements (Adh1 5′ MAR and ARS1) reduced transgene silencing but had no effect on the variability of expression. In transgenic plants, Adh1 5′ MAR had the effect of localizing β-glucuronidase expression to lateral root initiation sites. A possible model accounting for the function of Adh1 5′ MAR is discussed.

AB - Matrix attachment regions (MARs) are DNA sequences that bind an internal nuclear network of nonhistone proteins called the nuclear matrix. Thus, they may define discrete gene-containing chromatin loops in vivo. We have studied the effects of flanking transgenes with MARs on transgene expression levels in maize callus and in transformed maize plants. Three MAR elements, two from maize (Adh1 5′ MAR and Mha1 5′ MAR) and one from yeast (ARS1), had very different effects on transgene expression that bore no relation to their affinity for the nuclear matrix in vitro. In callus, two of the MAR elements (Adh1 5′ MAR and ARS1) reduced transgene silencing but had no effect on the variability of expression. In transgenic plants, Adh1 5′ MAR had the effect of localizing β-glucuronidase expression to lateral root initiation sites. A possible model accounting for the function of Adh1 5′ MAR is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0036743387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036743387&partnerID=8YFLogxK

U2 - 10.1105/tpc.004028

DO - 10.1105/tpc.004028

M3 - Article

VL - 14

SP - 2251

EP - 2264

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 9

ER -